Development of a lacZ marked WEHI-3B D(+) murine leukemic cell line as an in-vivo model of acute non-lymphocytic leukemia Journal Article
| Authors: | Smith, C.; Muench, M. O.; Knizewski, M.; Gilboa, E.; Moore, M. A. S. |
| Article Title: | Development of a lacZ marked WEHI-3B D(+) murine leukemic cell line as an in-vivo model of acute non-lymphocytic leukemia |
| Abstract: | Established leukemic cell lines have been useful models for studying the biology of leukemia. Analysis of the actions of differentiating agents on leukemic cell lines in vivo has been limited by an inability to unambiguously distinguish host hematopoietic elements from differentiated leukemic cells. In order to identify and quantify leukemic cells during in vivo studies, a derivative of the murine myelomonocytic leukemia cell line WEHI-3B D+, which stably expresses β-galactosidase, was constructed utilizing retroviral vector gene transfer. This cell line, termed WEHI-3B D+/lacZ 2.8, demonstrated in vitro growth and differentiation properties similar to the parental cell line. WEHI-3B D+/lacZ 2.8 expressed high levels of β-galactosidase following prolonged in vitro growth and following differentiation in suspension cultures and clonogenic assays. In vivo, WEHI-3B D+/lacZ 2.8 was leukemogenic and high level expression of β-galactosidase was maintained. Quantification of tissue involvement with WEHI-3B D+/lacZ 2.8 leukemia was performed utilizing staining with the fluorogenic β-galactosidase substrate fluorescein di-β-galactoside and fluorescence-activated cell sorting analysis. In vivo differentiation efficiency following granulocyte colony-stimulating factor (G-CSF) administration was determined using a simultaneous nuclear and cytoplasmic staining procedure. Results indicate that treatment of mice inoculated with WEHI-3B D+/lacZ 2.8 cells with G-CSF administration causes detectable but limited differentiation. |
| Keywords: | controlled study; nonhuman; sensitivity and specificity; animal cell; mouse; animal; mice; cell division; gene expression; cell growth; tumor markers, biological; fluorescent dye; animal experiment; animal model; cell differentiation; tumor cells, cultured; transfection; mice, inbred balb c; gene transfer; tissue distribution; leukemogenesis; escherichia coli; beta galactosidase; reporter gene; granulocyte colony stimulating factor; retroviridae; leukemia cell line; virus vector; beta-galactosidase; granulocyte-macrophage colony-stimulating factor; acute nonlymphocytic leukemia; myelomonocytic leukemia; tumor stem cell assay; fluorescence activated cell sorter; leukemic infiltration; male; female; priority journal; article; support, u.s. gov't, p.h.s.; leukemia, myelomonocytic, acute |
| Journal Title: | Leukemia |
| Volume: | 7 |
| Issue: | 2 |
| ISSN: | 0887-6924 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1993-02-01 |
| Start Page: | 310 |
| End Page: | 317 |
| Language: | English |
| PUBMED: | 8426483 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
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