Constitutive production of leukemia differentiation, colony-stimulating, erythroid burst-promoting, and pluripoietic factors by a human hepatoma cell line: Characterization of the leukemia differentiation factor Journal Article

  

Authors:	Gabrilove, J. L.; Welte, K.; Lu, L.; Castro-Malaspina, H.; Moore, M. A. S.
Article Title:	Constitutive production of leukemia differentiation, colony-stimulating, erythroid burst-promoting, and pluripoietic factors by a human hepatoma cell line: Characterization of the leukemia differentiation factor
Abstract:	Conditioned medium (CM) obtained from a human hepatoma cell line, SK-HEP-1, contains colony-stimulating factors (CSFs) active on murine and human bone marrow-derived granulocyte and macrophage colony-forming units (CFU-GM) and a factor capable of inducing granulocyte-macrophage differentiation (GM-DF) of murine myelomonocytic leukemic cells WEHI-3B(D+) and human promyelocytic leukemic cells HL-60 when assayed in semisolid agar cultures. The human active granulocyte-macrophage colony-stimulating factor (GM-CSF) for day 7 CFU-GM and the GM-DF for WEHI-3B(D+) and for HL-60 are not separable by acrylamide agarose column chromatography, eluting at an apparent molecular weight between 20,000 and 35,000 daltons, or by isoelectric focusing (isoelectric point, pH 5.4). In addition, SK-HEP-1 CM contains erythroid burst-promoting activity (BPA) and a factor that promotes the growth of human mixed colonies. SK-HEP-1 cells, which grow as an adherent monolayer, appear not to be endothelial or monocytic in origin since by immunofluorescent staining they are negative for Ia (HLA-DR), monocyte antigen 1 and 2, lysozyme, and factor VIII-related antigen. Positive immunofluorescent staining for keratin and fibronectin suggests the possibility that SK-HEP-1 is an epithelial cell line. Constitutive production of GM-DF as well as othe hematopoietic activities including GM-CSF, erythroid BPA, and an activity that promotes the growth of human mixed colony progenitors by a human epithelial tumor cell line, SK-HEP-1, suggests that this cell line is a valuable resource for both large-scale production of these factors and the cloning of the gene(s) that code for these regulators.
Keywords:	leukemia; human cell; in vitro study; liver; cell culture; differentiation; colony stimulating factor; hepatoma cell; burst forming unit e; human; priority journal
Journal Title:	Blood
Volume:	66
Issue:	2
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	1985-08-01
Start Page:	407
End Page:	415
Language:	English
DOI:	10.1182/blood.v66.2.407.407
PUBMED:	2990610
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021807964&doi=10.1182%2fblood.v66.2.407.407&partnerID=40&md5=805a54bfb0ad497f9e69a52f5fc3e136
Notes:	Article -- Export Date: 26 October 2021 -- Source: Scopus

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