Stimulation of human hematopoietic progenitor cell proliferation and differentiation by recombinant human interleukin 3. Comparison and interactions with recombinant human granulocyte-macrophage and granulocyte colony-stimulating factors Journal Article


Authors: Ottmann, O. G.; Abboud, M.; Welte, K.; Souza, L. M.; Pelus, L. M.
Article Title: Stimulation of human hematopoietic progenitor cell proliferation and differentiation by recombinant human interleukin 3. Comparison and interactions with recombinant human granulocyte-macrophage and granulocyte colony-stimulating factors
Abstract: Recombinant human interleukin 3 (IL3) produced in Escherichia coli was purified and its activities examined in cultures of highly enriched human bone marrow progenitor cells. Human IL3 stimulated multipotential (CFU-GEMM) and erythroid (BFU-E) progenitor cells, generating 95% more BFU-E than recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF). No further enhancement of BFU-E or CFU-GEMM occurred when IL3 and GM-CSF were used in combination. Human IL3 was more effective than GM-CSF in stimulating granulocyte-macrophage colony-forming cells (CFU-GM) in short-term suspension cultures, but did not induce an increase of CFU-GM, BFU-E, or CFU-GEMM above input levels. IL3 was more active on day-14 (d14) than on d7 CFU-GM, similar to GM-CSF, but generated fewer and smaller CFU-GM-derived clones than either GM-CSF or granulocyte CSF (G-CSF). The simultaneous addition of plateau levels of IL3 and GM-CSF resulted in an infra-additive augmentation of d7 and d14 CFU-GM-derived clones, whereas IL3 and G-CSF enhanced the number of cellularity predominantly of d14 CFU-GM. In liquid cultures, IL3 induced a > 100-fold increase in the number of basophil-mast-like cells and eosinophils and allowed maintenance of these cultures for up to 7 weeks. Human GM-CSF was an almost equally potent stimulus of eosinophil development but had only a marginal effect on basophilic precursors, whereas G-CSF lacked both activities. Therefore, human IL3 is a multilineage hemopoietic growth factor whose activities appear to encompass and extent beyond those of GM-CSF.
Keywords: adult; human cell; comparative study; cell division; bone marrow; erythropoiesis; granulocyte macrophage colony stimulating factor; cell differentiation; extracellular matrix; recombinant proteins; hematopoietic stem cells; hematopoiesis; granulocyte colony stimulating factor; cell separation; granulocyte colony-stimulating factor; drug interactions; colony-stimulating factors; growth substances; mast cells; granulocyte-macrophage colony-stimulating factor; eosinophils; interleukin 3; interleukin-3; granulopoiesis; suspensions; basophils; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Experimental Hematology
Volume: 17
Issue: 2
ISSN: 0301-472X
Publisher: Elsevier Science, Inc.  
Date Published: 1989-02-01
Start Page: 191
End Page: 197
Language: English
PUBMED: 2463933
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 14 April 2020 -- Source: Scopus
Citation Impact
MSK Authors
  1. Karl Welte
    59 Welte
  2. Louis M. Pelus
    27 Pelus