Specific neonatally induced tolerance to Mls locus determinants Journal Article
| Authors: | Macphail, S.; Ishizaka, S. T.; Bykowsky, M. J.; Lattime, E. C.; Stutman, O. |
| Article Title: | Specific neonatally induced tolerance to Mls locus determinants |
| Abstract: | Neonatal injection of CBA/HT6T6 [H-2(k), Mlsb] mice with adult, Mls-incompatible [CBA/J [H-2(k), Mls(d)] x CBA/HT6T6]F1 spleen cells results in the abrogation of cell proliferation and interleukin 2 (IL 2) production in bulk mixed lymphocyte cultures, when spleen cells from the inoculated mice are tested at 6 to 8 wk of age with stimulator cells expressing the Mls(d) of the tolerizing inoculum. In limiting dilution assays, this tolerant state was manifested in a 25- to 550-fold (280-fold average) decrease in the frequency of precursors of Mls(d)-responsive IL 2-producing T cells. Tolerance was specific in that the frequencies of precursors of IL 2-producing cells responding to Con A, allogeneic H-2(d), and self-Ia were not affected. The observed low frequency of Mls-responsive cells was due neither to extensive chimerism resulting in the dilution of Mls(d)-responsive cells by the nonresponsive F1 cells of the inoculum, nor to the action of suppressor cells. These findings indicate that neonatal injection of Mls-incompatible spleen cells produces a state of specific tolerance by a clonal deletion or inactivation mechanism. This specific tolerance supports the view that 1) the Mls locus encodes or regulates the expression of defined alloantigenic determinants and 2) Mls-incompatible responder mice have specific receptors for Mls determinants on clonally distributed IL 2-producing responder T cells. |
| Keywords: | nonhuman; lymphocyte proliferation; t lymphocyte; mouse; animals; mice; interleukin 2; spleen; in vitro study; mice, inbred balb c; chimera; immunological tolerance; lymphocyte activation; immune tolerance; t-lymphocytes, regulatory; stem cells; leukocyte count; animals, newborn; mice, inbred cba; lymphocytes; mixed lymphocyte reaction; interleukin-2; spleen cell; epitopes; lymphocyte transfusion; histocompatibility antigens; priority journal |
| Journal Title: | Journal of Immunology |
| Volume: | 135 |
| Issue: | 5 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1985-11-01 |
| Start Page: | 2967 |
| End Page: | 2974 |
| Language: | English |
| PROVIDER: | scopus |
| DOI: | 10.4049/jimmunol.135.5.2967 |
| DOI/URL: | |
| Notes: | DOI currently broken -- Source: Scopus |
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