| Abstract: |
The studies described show that functional Mls specific tolerance, which we previously reported in peripheral spleen cells of mice injected within 24 h of birth with Mls incompatible spleen cells, is observable in the thymus on day 6. At this time a significant positive response is not detectable in spleen cells of normal mice. In the limiting dilution assay, we are able to detect a more profound depletion than others have found with anti-TCR antibodies. The tolerance in the thymus is not due to active suppression or simple dilution of responders by nonresponsive cells of the neonatal inoculum. By tolerizing BALB/c (Mls(b,c)) mice with spleen cells from Mlsa congenic mice, we show that Mlsa incompatibility alone is sufficient for tolerance induction. Data from these experiments also show that the T cells seen responding at high frequency to stimulators from mice expressing Mlsa determinants, as well as many other non-H-2 encoded incompatibilities, are indeed responding to Mlsa determinants. In addition, experiments involving neonatal injection of Mlsb mice with Mlsa and Mls(c) spleen cells show no crossreactivity of tolerance between Mlsa and Mls(c) haplotypes. Our findings also show coexpression of determinants common to both Mlsa and Mls(c) haplotypes by the Mls(d) haplotype. In all, the described experiments elucidate a pattern of Mls determinant specific hyporesponsiveness, in mice neonatally injected with appropriate allogeneic spleen cells, which bears all the hallmarks of functional, alloantigen specific, clonal deletion type tolerance. |
| Keywords: |
nonhuman; t-lymphocytes; proteins; animal cell; mouse; animal; mice; spleen; animal experiment; animal model; haplotypes; mice, inbred balb c; haplotype; immunological tolerance; immune tolerance; cell culture; thymus; thymus gland; newborn; animals, newborn; mice, inbred cba; alloantigen; isoantigens; antigens, surface; spleen cell; solubility; mice, inbred c3h; epitopes; mice, inbred dba; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; mice, inbred akr; minor lymphocyte stimulatory antigens
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