| Abstract: |
The level of cell proliferation and interleukin-2 (IL-2) production observed in an anti-Mls mixed lymphocyte reaction between spleen cells from H-2 compatible, Mls incompatible mouse strains is determined by the H-2 haplotype of the mouse combination. Thus, while AKR (H-2k) spleen cells stimulated strong M1sa responses in H-2k responder cells, AKR H-2b spleen cells stimulated no or negligible M1sa responses in responder cells from H-2bmouse strains. This effect was observed at the levels of IL-2 production and cell proliferation. The magnitude of the response observed using F1 (H-2k/H-2b) responder cells was found to be a function of stimulator rather than responder cells. The poor stimulatory capacity of AKR H-2bspleen cells was also shown not to be due to the loss of the stimulatory Mlsaallele during the construction of the congenic strain from AKR and C57BL/6 parental strains. Using stimulator cells from a second series of congenic mice, we found H-2b(strain DLLP) again to represent a poorly Mlsa stimulatory H-2 haplotype. In addition, H-2q (DBA/1) cells displayed very poor Mlsa stimulatory potential while H-2d (D1.C) cells were efficient Mlsa stimulators. Again the effect was shown to be at the level of the stimulator cells. In toto, our findings indicate that the H-2kand H-2dhaplotypes encode strong Mlsa stimulatory potential while the H-2band H-2qhaplotypes determine poor Mlsa stimulatory potential in primary in vitro responses, measured as cell proliferation and IL-2 production. © 1986 Springer-Verlag. |
| Keywords: |
nonhuman; animal cell; mouse; animal; mice; cell division; heredity; interleukin 2; gene expression; cell growth; spleen; alleles; mice, inbred strains; lymphocyte culture test, mixed; gene control; lymphocytes; mixed lymphocyte reaction; interleukin-2; spleen cell; lymphocyte transformation; reticuloendothelial system; h-2 antigens; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; h2 system; blood and hemopoietic system
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