Assignment of human nerve growth factor receptor gene to chromosome 17 and regulation of receptor expression in somatic cell hybrids Journal Article
| Authors: | Rettig, W. J.; Thomson, T. M.; Spengler, B. A.; Biedler, J. L.; Old, L. J. |
| Article Title: | Assignment of human nerve growth factor receptor gene to chromosome 17 and regulation of receptor expression in somatic cell hybrids |
| Abstract: | Nerve growth factor (NGF) is a polypeptide hormone which plays a central role in the development and growth of sympathetic and sensory neurons. The effects of NGF on target cells are mediated by a specific cell surface structure, nerve growth factor receptor (NGFr), which has been identified in human cells as a 75,000-mol-wt glycoprotein. We have used a monoclonal antibody to human NGFr to study cell-surface expression of the receptor on a panel of mouse-human neuroblastoma hybrids, and the serological typing results permit assignment of the gene coding for NGFr (NGFR) to chromosome 17q21-qter. In addition to mouse-human neuroblastoma hybrids, human NGFr was also detected on hybrids derived from fusions between mouse L-cell fibroblasts and human neuroblastoma and melanoma cells. Furthermore, induction of human NGFr expression was observed in hybrids derived from NGFr- human kidney epithelial cells and mouse L cells, but not in hybrids derived from human kidney epithelial cells and mouse RAG kidney carcinoma cells. These results suggest that cell-surface expression of human NGFr is controlled by transacting regulatory signals. © 1986 Plenum Publishing Corporation. |
| Keywords: | human cell; cytology; animals; mice; somatic cell; heredity; nerve growth factors; in vitro study; antibodies, monoclonal; genetic engineering; neuroblastoma; gene control; hormone; chromosome 17q; chromosome mapping; receptors, cell surface; nervous system; chromosomes, human, pair 17; receptors, nerve growth factor; cell receptor; hybrid cells; nerve growth factor; normal value; humans; human; gene assignment; cell hybridization |
| Journal Title: | Somatic Cell and Molecular Genetics |
| Volume: | 12 |
| Issue: | 5 |
| ISSN: | 0740-7750 |
| Publisher: | Springer New York LLC |
| Date Published: | 1986-09-01 |
| Start Page: | 441 |
| End Page: | 447 |
| Language: | English |
| DOI: | 10.1007/bf01539915 |
| PUBMED: | 3020711 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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