Monoclonal antibody to human Trk-A: Diagnostic and therapeutic potential in neuroblastoma Conference Paper

Authors: Kramer, K.; Gerald, W.; Lesauteur, L.; Saragovi, H. U.; Cheung, N. K. V.
Title: Monoclonal antibody to human Trk-A: Diagnostic and therapeutic potential in neuroblastoma
Conference Title: Advances in Neuroblastoma Research - 1996 (ANR 6)
Abstract: 5C3 is a murine IgG1 antibody specific for the nerve growth factor (NGF) docking site of the human p140 trk-A receptor, with no cross-reactivity with human trk-B. In vitro, 5C3 and its Fab mimic the effects of NGF, a neurotrophin mediating growth and differentiation of neural crest-derived cells. When labelled with radioisotope, 5C3 images human trk-A positive tumours in vivo. More importantly, 5C3 induces regression of human trk-A positive tumours in rodents. We therefore investigated the value of 5C3 in detecting trk-A expression in human neuroblastoma by immunohistochemistry. 5C3 reactivity was detected in 73 of 113 neuroblastoma specimens and correlated strongly with localised/4s disease (55/60) with either a homogeneous or mixed pattern. Among stage 4 neuroblastoma, only 18/53 had homogeneous or mixed trk-A expression. 5C3 did not react with 46/48 other human malignancies, but was positive in 1 melanoma and 1 Wilms' tumour specimen. The prognostic, imaging and NGF-mimetic properties of antibody 5C3 and its derivatives may offer alternatives for the diagnosis and treatment of neuroblastoma.
Keywords: immunohistochemistry; human tissue; major clinical study; conference paper; cancer staging; cancer immunotherapy; gene expression; neoplasm proteins; childhood cancer; monoclonal antibody; antibodies, monoclonal; infant; neuroblastoma; tyrosine kinase receptor; antibody specificity; immunoglobulin g1; immunoglobulin g antibody; nerve growth factor receptor; neurotrophin receptor; receptor, trka; humans; prognosis; human; priority journal; p140 trk-a nerve growth factor receptor; paediatric malignancies; trk-a
Journal Title European Journal of Cancer
Volume: 33
Issue: 12
Conference Dates: 1996 May 22-25
Conference Location: Philadelphia, PA
ISBN: 0959-8049
Publisher: Elsevier Inc.  
Date Published: 1997-10-01
Start Page: 2090
End Page: 2091
Language: English
DOI: 10.1016/s0959-8049(97)00210-4
PUBMED: 9516859
PROVIDER: scopus
Notes: Conference Paper -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Nai-Kong Cheung
    439 Cheung
  2. Kim Kramer
    168 Kramer
  3. William L Gerald
    367 Gerald