Cell-surface antigens determined by human chromosomes 1 and 12: Comparative serological and somatic cell genetic analysis of eight antigenic systems Journal Article

  

Authors:	Rettig, W. J.; Dracopoli, N. C.; Spengler, B. A.; Kersey, J. H.; Biedler, J. L.; Old, L. J.
Article Title:	Cell-surface antigens determined by human chromosomes 1 and 12: Comparative serological and somatic cell genetic analysis of eight antigenic systems
Abstract:	We have previously assigned several genes controlling expression of cell-surface antigens to human chromosomes 1 and 12. In the present study, we characterize three additional cell-surface antigens determined by these chromosomes. Two monoclonal antibodies, AbSR75 and AbMG6, define antigens expressed on a wide range of cultured human cells. In contrast, AbK66 defines an antigen with a more restricted distribution which is expressed on normal and malignant human epithelial cells but not on neuroectoderm-derived cells or hematopoietic cells. Normal adult fibroblasts are K66-, whereas fetal fibroblasts are K66+. Serological analysis of rodent-human hybrid cells permitted the assignment of MSK31, controlling SR75, and MSK32, controlling K66, to chromosome 1, whereas MSK27, controlling MG6, maps to chromosome 12. Analysis of hybrids containing only deleted copies of chromosome 1 or chromosome 12 established regional assignments for MSK31, MSK32, and MSK27 and also for the previously defined genes MSK1, MSK4, and MSK7. The MSK1 gene, coding for a 140,000 mol wt cell surface glycoprotein, was found to map to the same band (1p22) as the NRAS protooncogene and the gene encoding the β-subunit of nerve growth factor (NGFB). © 1986 Plenum Publishing Corporation.
Keywords:	human cell; chromosome 1; chromosome 12; animals; mice; heredity; cell line; in vitro study; histology; monoclonal antibody; gene mapping; membrane antigen; antibodies, monoclonal; rodentia; genetic markers; gene location; antigens, surface; chromosome mapping; hybrid cell; hybrid cells; chromosome banding; chromosomes, human, 16-18; normal value; serotyping; humans; human; gene assignment; chromosomes, human, 1-3
Journal Title:	Somatic Cell and Molecular Genetics
Volume:	12
Issue:	1
ISSN:	0740-7750
Publisher:	Springer New York LLC
Date Published:	1986-01-01
Start Page:	33
End Page:	40
Language:	English
DOI:	10.1007/bf01560725
PUBMED:	3456173
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022633170&doi=10.1007%2fBF01560725&partnerID=40&md5=8f3c5e0c467449aee68e9596255090c1
Notes:	Article -- Export Date: 18 August 2021 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/185846