Localisation on human chromosome 19 of three genes for cell surface antigens defined by monoclonal antibodies Journal Article
| Authors: | Brook, J. D.; Beresford, H. R.; Shaw, D. J.; Old, L. J.; Rettig, W. J. |
| Article Title: | Localisation on human chromosome 19 of three genes for cell surface antigens defined by monoclonal antibodies |
| Abstract: | Expression of three distinct human cell surface antigens defined by monoclonal antibodies (mAbs) was examined in a series of rodent-human somatic cell hybrids retaining different subsets of human chromosomes. Cell surface reactivity with mAbs F8 and G253, detecting a 95 kilodalton (kD) glycoprotein (gp95); with mAbs F10 and A103, detecting a 50 kD glycoprotein (gp50); and with mAb S7 was found to cosegregate with human chromosome 19. However, differential antigen expression was observed with hybrids containing fragments of the 19 and hybrids constructed with different human cell types. Comparison of results from the serological typing with the presence of a number of chromosome 19 DNA markers in hybrid cells and cytogenetic analysis suggests that MSK20, the gene coding for the F10/A103 antigen gp50, is located in chromosome region 19pter-”19pl3.2. The genes coding for the F8/G253 antigen, gp95 (gene symbol MSK19) and the S7 antigen (MSK37) are located in region 19p 13.2-* 19q 13.2. Thus, the cell surface antigens described in this study may be used as selectable markers for specific portions of human chromosome 19. © 1987 S. Karger AG, Basel. |
| Keywords: | human cell; animal cell; chromosome 19; animal; heredity; cell line; breast neoplasms; monoclonal antibody; membrane antigen; antibodies, monoclonal; genetic engineering; cell culture; chromosomes, human, pair 19; radioisotope; lymphocytes; astrocytoma; cytochemistry; clone cells; gene location; normal human; antigens, surface; chromosome mapping; southern blotting; hybrid cell; hybrid cells; normal value; human; female; support, non-u.s. gov't; dna probe; gene assignment; genes, structural |
| Journal Title: | Cytogenetics and Cell Genetics |
| Volume: | 45 |
| Issue: | 3-4 |
| ISSN: | 0301-0171 |
| Publisher: | S. Karger AG |
| Date Published: | 1987-01-01 |
| Start Page: | 156 |
| End Page: | 162 |
| Language: | English |
| DOI: | 10.1159/000132447 |
| PUBMED: | 3691180 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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