Genes controlling gp25/30 cell-surface molecules map to chromosomes X and Y and escape X-inactivation Journal Article
| Authors: | Dracopoli, N. C.; Rettig, W. J.; Albino, A. P.; Esposito, D.; Archidiacono, N.; Rocchi, M.; Siniscalco, M.; Old, L. J. |
| Article Title: | Genes controlling gp25/30 cell-surface molecules map to chromosomes X and Y and escape X-inactivation |
| Abstract: | The monoclonal antibody AbO13 defines a cell-surface antigen that is expressed on most cultured human cells, but not on rodent cells. AbO13 precipitates glycoproteins of 25,000 and 30,000 mol. wt. from lysates of [3H]glucosamine-labeled human cells. Results of the serological typing of a panel of 25 rodent-human somatic cell hybrid clones show that reactivity with AbO13 segregates with the human X and Y chromosomes. The presence of either of these chromosomes is sufficient for O13 expression on the hybrid cell surface. Analysis of hybrid clones containing human X chromosomes with karyotypically defined deletions permitted the regional assignment of the X-linked gene locus controlling the expression of O13 to Xp22-pter. In addition, AbO13 is reactive with Chinese hamster-human hybrids derived from fibroblasts of a 49,XXXXX individual that contained only inactivated copies of the human X chromosome. These results suggest that the X-linked locus determining the expression of O13 is not subject to X-inactivation. |
| Keywords: | human cell; animals; heredity; cell line; abo blood-group system; in vitro study; histology; monoclonal antibody; membrane antigen; antibodies, monoclonal; x chromosome; x chromosome inactivation; dosage compensation, genetic; rodentia; radioisotope; karyotyping; molecular weight; glycoproteins; gene location; normal human; antigens, surface; chromosome mapping; y chromosome; cricetinae; cricetulus; cricetulus griseus; hybrid cell; hybrid cells; chromosome banding; normal value; humans; human; male; female; priority journal |
| Journal Title: | American Journal of Human Genetics |
| Volume: | 37 |
| Issue: | 1 |
| ISSN: | 0002-9297 |
| Publisher: | Cell Press |
| Date Published: | 1985-01-01 |
| Start Page: | 199 |
| End Page: | 207 |
| Language: | English |
| PUBMED: | 4038849 |
| PROVIDER: | scopus |
| PMCID: | PMC1684553 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
