Loss of heterozygosity at autosomal and X-linked loci during tumor progression in a patient with melanoma Journal Article
| Authors: | Dracopoli, N. C.; Alhadeff, B.; Houghton, A. N.; Old, L. J. |
| Article Title: | Loss of heterozygosity at autosomal and X-linked loci during tumor progression in a patient with melanoma |
| Abstract: | Restriction fragment length polymorphisms at 61 autosomal and 7 X-linked loci were screened for heterozygosity in cell lines derived from 6 independent metastases and autologous B-cells from a patient with melanoma. Segregations resulting in the loss of heterozygosity were detected in the tumor cells at 8 of 16 autosomes with at least 1 informative locus and at the 3 informative X-linked loci. With a single exception, karyotypic abnormalities were not detected in the region of loci where loss of heterozygosity had been detected. Three patterns of loss were identified: (a) unique segregations in cells from a single metastasis; (b) segregation of the same alleles in different subsets of metastases; and (c) identical segregations in all 6 metastases. The monoclonal derivation of the 6 metastases is supported by the inactivation of the same X-chromosome and the presence of identical segregations at loci on chromosomes 9 and X. Analysis of the patterns of segregation in the metastatic tumor cells permitted the development of a genealogy of tumor progression in this patient and the development of a model of tumor progression which describes the accumulation of selectively neutral and advantageous segregations in metastatic tumor cells. © 1987, American Association for Cancer Research. All rights reserved. |
| Keywords: | aged; case report; comparative study; melanoma; models, biological; skin neoplasms; cell line; b-lymphocytes; selection (genetics); heterozygosity; chromosome aberrations; tumor growth; aneuploidy; clone cells; genetic markers; abdominal neoplasms; polymorphism, restriction fragment length; human; female; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; dosage compensation (genetics) |
| Journal Title: | Cancer Research |
| Volume: | 47 |
| Issue: | 15 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1987-08-01 |
| Start Page: | 3995 |
| End Page: | 4000 |
| Language: | English |
| PUBMED: | 2886213 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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