Loss of polymorphic restriction fragments in malignant melanoma: Implications for tumor heterogeneity Journal Article

  


Authors: Dracopoli, N. C.; Houghton, A. N.; Old, L. J.
Article Title: Loss of polymorphic restriction fragments in malignant melanoma: Implications for tumor heterogeneity
Abstract: Loss of genetic material at certain chromosomal sites is implicated in the etiology of retinoblastoma and Wilms tumor. Whether specific chromosomal deletions are associated with other types of human cancer needs to be explored. We have examined 24 melanoma cell lines, derived from 21 patients with nonfamilial malignant melanoma, for evidence of somatically induced hemizygosity or homozygosity. Twelve DNA probes, recognizing single-copy restriction fragment length polymorphisms (RFLP) determined by loci on 11 different chromosomes, were used to screen autologous combinations of melanoma cells and either B cells or fibroblasts. Loss of heterozygosity in melanoma cells was identified at 27 of 100 informative loci. These losses occurred at loci on 8 different chromosomes, and the frequency of loss at individual loci varied between 8% and 67%. We conclude that somatic mutations resulting in homozygosity or hemizygostiy are common in melanoma and evidently not restricted to specific chromosomes.
Keywords: human cell; somatic mutation; heredity; melanoma; in vitro study; genetic engineering; radioisotope; chromosome deletion; dna polymorphism; etiology; restriction fragment length polymorphism; human; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 82
Issue: 5
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1985-03-01
Start Page: 1470
End Page: 1474
Language: English
DOI: 10.1073/pnas.82.5.1470
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021916368&doi=10.1073%2fpnas.82.5.1470&partnerID=40&md5=d83ff8fbf988528f3a4b157df94b13e8
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Alan N Houghton
    364 Houghton
  2. Lloyd J Old
    593 Old
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