Bovine steroid 21-hydroxylase: Regulation of biosynthesis Journal Article
| Authors: | John, M. E.; Okamura, T.; Dee, A.; Adler, B.; John, M. C.; White, P. C.; Simpson, E. R.; Waterman, M. R. |
| Article Title: | Bovine steroid 21-hydroxylase: Regulation of biosynthesis |
| Abstract: | A recombinant cDNA clone, PBC21-1, specific for bovine steroid 21-hydroxylase cytochrome P-450 (p-450c21)was identified in a bovine adrenocortical cDNA library, and this identity was confirmed by nucleotide sequencing which revealed significant amino acid homology (77%) with human P-450C21Cdna. The pBC21-1 insert is 1.7 kilobases in length and includes a 1128 base pair region that encodes the C-terminal 376 amino acids of bovine P-450C21 As well as 535 base pairs of 3'-untranslated sequence. A novel feature of this insert is a 20 base pair intervening sequence near the 5' end, apparently the result of an aberrant splicing event. Northern blot analysis reveals that bovine P-450C21 is encoded by two transcripts, 2.3 and 2.0 kilobases in length which are detected in adrenal cortical RNA. Bovine liver, heart, kidney, and corpus luteum do not contain detectable P-450C21 transcripts. Regulation of P-450C21 gene expression by adrenocorticotropin was investigated with pBC21-l and bovine adrenocortical cells in primary, monolayer culture. Treatment with ACTH or analogues of cAMP increases the steady-state levels of P-450C21RNA in such cell cultures. In vitro transcription run-on assays suggest that this increase is, at least in part, due to the enhanced transcriptional activity of the P-450C21 gene. © 1986, American Chemical Society. All rights reserved. |
| Keywords: | nonhuman; animal cell; animalia; liver; kidney; cattle; heart; complementary dna; cytochrome p450; female genital system; endocrine system; corpus luteum; bovinae; bos taurus; adrenal cortex; recombinant dna; priority journal; steroid 21 monooxygenase |
| Journal Title: | Biochemistry |
| Volume: | 25 |
| Issue: | 10 |
| ISSN: | 0006-2960 |
| Publisher: | American Chemical Society |
| Date Published: | 1986-05-20 |
| Start Page: | 2846 |
| End Page: | 2853 |
| Language: | English |
| DOI: | 10.1021/bi00358a016 |
| PROVIDER: | scopus |
| PUBMED: | 2424492 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: We appreciate the technical assistance of Leticia Cortez and Bill Rainey in the preparation of cell cultures. We thank Walter L. Miller, Department of Pediatrics, University of California, San Francisco, for providing unpublished information on intronic and exonic sequences near the 5' end of bovine P-450C2l gene and D. Russell and T. Yamamoto of this institution for providing a bovine adrenocortical cDNA library. We also thank Dr. E. F. Johnson for providing the sequence of rabbit liver P-450 form 1 prior to its publication. |
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