Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes Journal Article

   


Authors: Trapani, J. A.; Klein, J. L.; White, P. C.; Dupont, B.
Article Title: Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes
Abstract: A cDNA clone encoding a human serine esterase gene was isolated from a library constructed from poly(A)+ RNA of allogeneically stimulated, interleukin 2-expanded peripheral blood mononuclear cells. The clone, designated HSE26.1, represents a full-length copy of a 0.9-kilobase mRNA present in human cytotoxic cells but absent from a wide variety of noncytotoxic cell lines. Clone HSE26.1 contains an 892-base-pair sequence, including a single 741-base-pair open reading frame encoding a putative 247-residue polypeptide. The first 20 amino acids of the polypeptide form a leader sequence. The mature protein is predicted to have an unglycosylated M(r) of ≃26,000 and contains a single potential site for N-linked glycosylation. The nucleotide and predicted amino acid sequences of clone HSE26.1 are homologous with all murine and human serine esterases cloned thus far but are most similar to mouse granzyme B (70% nucleotide and 68% amino acid identity). HSE26.1 protein is expressed weakly in unstimulated peripheral blood mononuclear cells but is strongly induced within 6-hr incubation in medium containing phytohemagglutinin. The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity.
Keywords: human cell; molecular cloning; serine proteinase; genetic engineering; cell culture; murinae; natural killer cell; cell mediated cytotoxicity; cytotoxic lymphocyte; human; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 85
Issue: 18
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1988-09-01
Start Page: 6924
End Page: 6928
Language: English
DOI: 10.1073/pnas.85.18.6924
PUBMED: 3261871
PROVIDER: scopus
PMCID: PMC282091
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023688825&doi=10.1073%2fpnas.85.18.6924&partnerID=40&md5=ee113181cb367751fe4ec575f68a7555
Notes: Article -- Export Date: 6 August 2020 -- Source: Scopus
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MSK Authors
  1. Bo Dupont
    264 Dupont
  2. Joseph A. Trapani
    16 Trapani
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