Cloning of the cDNA encoding phenylalanyl tRNA synthetase regulatory α-subunit-like protein whose expression is down-regulated during differentiation Journal Article
| Authors: | Zhou, X.; Richon, V. M.; Ngo, L.; Rifkind, R. A.; Marks, P. A. |
| Article Title: | Cloning of the cDNA encoding phenylalanyl tRNA synthetase regulatory α-subunit-like protein whose expression is down-regulated during differentiation |
| Abstract: | Hybrid polar compounds (HPCs), such as suberoylanilide hydroxamic acid (SAHA), induce differentiation of transformed cells. Differential display of RNA was used to identify genes whose expression is changed during SAHA-induced differentiation of murine erythroleukemia (MEL) cells. One such cDNA was identified whose mRNA level decreased by 50% after 8 h of SAHA treatment as determined by Northern blot analysis. The full-length cDNA (1944 bp in length) was cloned by sequencing of an EST clone and rapid amplification of 5' cDNA ends (5'-RACE). The predicted amino acid sequence is 589 amino acids and shares 45% identity with the yeast cytoplasmic phenylalanyl tRNA synthetase (PheRS) regulatory α-subunit. Human EST clones which share over 90% identity of predicted amino acid sequence with this cDNA map to chromosome 2, near the paired box homeotic gene 3 (PAX3) locus, a region syngenic to mouse chromosome 1. This is the first report of the cloning of the full-length cDNA for the murine PheRS regulatory α-subunit-like protein. The level of PheRS α-subunit-like mRNA is regulated during differentiation but not during cell cycle progression. |
| Keywords: | nonhuman; animal cell; chromosome 1; mouse; mammalia; animals; mice; cell cycle; gene amplification; down-regulation; cell differentiation; tumor cells, cultured; animalia; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; sequence homology, amino acid; messenger rna; cell transformation; nucleotide sequence; murinae; cytoplasm; base sequence; down regulation; rodentia; yeast; sequence homology; enzyme subunit; chromosome 2; complementary dna; dna, complementary; northern blotting; hydroxamic acid derivative; homeobox; saha; erythroleukemia; humans; human; priority journal; article; erythroleukemia cell; hybrid polar compound; phenylalanine transfer rna ligase; phenylalanine-trna ligase |
| Journal Title: | Gene |
| Volume: | 233 |
| Issue: | 1-2 |
| ISSN: | 0378-1119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1999-06-11 |
| Start Page: | 13 |
| End Page: | 19 |
| Language: | English |
| DOI: | 10.1016/s0378-1119(99)00170-5 |
| PUBMED: | 10375616 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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