Cloning of the human orphan receptor germ cell nuclear factor/retinoid receptor-related testis-associated receptor and its differential regulation during embryonal carcinoma cell differentiation Journal Article
| Authors: | Lei, W.; Hirose, T.; Zhang, L. X.; Adachi, H.; Spinella, M. J.; Dmitrovsky, E.; Jetten, A. M. |
| Article Title: | Cloning of the human orphan receptor germ cell nuclear factor/retinoid receptor-related testis-associated receptor and its differential regulation during embryonal carcinoma cell differentiation |
| Abstract: | We have cloned a cDNA encoding the full-length coding region of the human homologue of the germ cell nuclear factor (GCNF)/retinoid receptor-related testis-associated receptor (RTR), from a human testis cDNA library. The amino acid sequence of human GCNF/RTR is highly homologous to that of the mouse GCNF/RTR. The largest difference between the two homologues is a 15 amino acid deletion in the human GCNF/RTR at amino acid 47. The GCNF/RTR gene was localized on human chromosome 9. Northern blot analysis using poly(A)+ RNA from different human tissues showed that GCNF/RTR mRNA is most abundantly expressed in the testis. GCNF/RTR was also highly expressed in embryonic stem cells and embryonal carcinoma cells but repressed in its differentiated derivatives. Induction of differentiation of mouse embryonal carcinoma F9 cells and human embryonal carcinoma NTERA-2 clone D1 (NT2/D1) cells by all-trans retinoic acid was accompanied by a down-regulation of GCNF/RTR. Our observations suggest that GCNF/RTR plays a role in the control of gene expression in early embryogenesis and during spermatogenesis. |
| Keywords: | controlled study; unclassified drug; human cell; dna-binding proteins; cytology; animals; mice; embryo development; cell differentiation; cloning, molecular; amino acid sequence; molecular sequence data; rna, messenger; stem cells; base sequence; molecular biology; retinoic acid; chromosome mapping; dna, complementary; receptors, cytoplasmic and nuclear; cloning; spermatogenesis; receptors, retinoic acid; tumor stem cells; humans; human; male; priority journal; article; retinoid receptor |
| Journal Title: | Journal of Molecular Endocrinology |
| Volume: | 18 |
| Issue: | 2 |
| ISSN: | 0952-5041 |
| Publisher: | BioScientifica Ltd. |
| Date Published: | 1997-04-01 |
| Start Page: | 167 |
| End Page: | 176 |
| Language: | English |
| PUBMED: | 9134503 |
| PROVIDER: | scopus |
| DOI: | 10.1677/jme.0.0180167 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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