| Abstract: |
Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans. |
| Keywords: |
controlled study; unclassified drug; major clinical study; neoplasms; animals; mice; disease association; reverse transcription polymerase chain reaction; breast cancer; gene expression; gene expression profiling; lung cancer; bladder cancer; tumor antigen; gene mapping; amino acid sequence; molecular sequence data; sequence homology, amino acid; antigens, neoplasm; tissue distribution; messenger rna; colon cancer; immunogenicity; nucleotide sequence; carrier proteins; antibody response; base sequence; liver cancer; antibodies, neoplasm; testis; swine; chromosome 12p; complementary dna; chromosome mapping; dna, complementary; protein precursors; southern blotting; gene isolation; chromosomes, human, pair 12; blotting, southern; protein precursor; humans; human; male; female; priority journal; article; sus scrofa; cavia porcellus; pcr cloning; testis cdna; proacrosin binding protein sp32 precursor
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