Serological analysis of BALB/c methylcholanthrene sarcoma Meth A by SEREX: Identification of a cancer/testis antigen Journal Article

Authors: Ono, T.; Sato, S.; Kimura, N.; Tanaka, M.; Shibuya, A.; Old, L. J.; Nakayama, E.
Article Title: Serological analysis of BALB/c methylcholanthrene sarcoma Meth A by SEREX: Identification of a cancer/testis antigen
Abstract: Antigens of BALB/c methylcholanthrene-induced fibrosarcoma Meth A recognized by the host humoral immune response were investigated by serological analysis of antigens by recombinant expression cloning (SEREX). Immunoscreening a cDNA library from Meth A (Kγ) cells (Meth A retrovirally transfected with murine IFN-γ cDNA) with sera from BALB/c mice growing parental Meth A transplants identified 10 antigens. One of them, OY-MS-4, showed characteristics of a cancer/testis (CT) antigen. Nucleotide sequence analysis revealed that OY-MS-4 was identical to a mouse placenta and embryonic expression gene (pem) known to be selectively expressed during embryogenesis and in transformed cell lines. In adult mice, expression of OY-MS-4 was restricted to testis and placenta. Four of 6 methylcholanthrene-induced fibrosarcomas in BALB/c mice showed strong expression of OY-MS-4. In 6 T-cell leukemias, only a dimethylbenzanthracene-induced leukemia, EL4. (C57BL), showed strong expression. Two other tumors, A20.2J and P815, induced by ethylnitrosourea and methylcholanthrene, respectively, also strongly expressed OY-MS-4. The other 9 gene products identified in Meth A by SEREX were expressed in all 15 tumors tested and in a range of normal tissues. Sequence analysis of cDNA inserts coding for the SEREX-defined antigens showed no evidence of mutation. Despite the expression of OY-MS-I-10 antigens in methylcholanthrene sarcomas other than Meth A, no antibody was detected in the sera of mice bearing these other sarcomas. The basis for the unique immunogenicity of OY-MS-I-10 presented by Meth A, but not by other syngeneic tumors expressing these gene products, is unknown. (C) 2000 Wiley-Liss, Inc.
Keywords: controlled study; sequence analysis; nonhuman; mouse; animals; mice; animal tissue; animal experiment; animal model; gene product; tumor cells, cultured; tumor antigen; mice, inbred balb c; amino acid sequence; molecular sequence data; fibrosarcoma; antigens, neoplasm; reverse transcriptase polymerase chain reaction; rna, messenger; immunogenicity; antigen recognition; mouse strain; t cell leukemia; serology; antibodies, neoplasm; testis; ethylnitrosourea; placenta; dna, complementary; carcinogens; antigen-antibody reactions; sarcoma, experimental; methylcholanthrene; 3 methylcholanthrene; serologic tests; male; priority journal; article; dimethylbenz[a]anthracene
Journal Title: International Journal of Cancer
Volume: 88
Issue: 6
ISSN: 0020-7136
Publisher: John Wiley & Sons  
Date Published: 2000-12-15
Start Page: 845
End Page: 851
Language: English
DOI: 10.1002/1097-0215(20001215)88:6<845::aid-ijc1>;2-n
PUBMED: 11093803
PROVIDER: scopus
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Lloyd J Old
    593 Old