Identification of the gonad-specific anion transporter SLCO6A1 as a cancer/testis (CT) antigen expressed in human lung cancer Journal Article

Authors: Lee, S. Y.; Williamson, B.; Caballero, O. L.; Chen, Y. T.; Scanlan, M. J.; Ritter, G.; Jongeneel, C. V.; Simpson, A. J.; Old, L. J.
Article Title: Identification of the gonad-specific anion transporter SLCO6A1 as a cancer/testis (CT) antigen expressed in human lung cancer
Abstract: Serological analysis of recombinant cDNA expression libraries (SEREX) has led to the identification of many of the antigens recognized by the immune system of cancer patients, which are collectively referred to as the cancer immunome. We used SEREX to screen a testicular cDNA expression library with sera obtained from non-small cell lung cancer patients and isolated cDNA clones for 82 antigens. These included a total of 31 antigens previously identified by SEREX, and 51 that did not match entries in the Cancer Immunome Database and were considered newly identified antigens. Overall, the antigens comprised 62 known proteins and 20 uncharacterized gene products. Six antigens (NY-TLU-6, -37, -39, -57, -70, -75) were identified as putative cell surface proteins that are potential targets for monoclonal antibody-based immunotherapy. Of these, the gonad-specific anion transport protein SLCO6A1 (NY-TLU-57) was shown to be tissue-restricted. RT-PCR showed it to be expressed strongly only in normal testis, and weakly in spleen, brain, fetal brain, and placenta. In addition, NY-TLU-57 mRNA was found in lung tumor samples (5/10) and lung cancer cell lines (6/11), as well as bladder (5/12) and esophageal (5/12) tumor samples. These data suggest that SLCO6A1 is a putative cancer/testis (CT) cell surface antigen of potential utility as a target for antibody-based therapy for a variety of tumor types. The analysis also permits us to estimate the eventual size of the SEREX-defined cancer immunome at around 4000 genes. This emphasizes the importance of continued SEREX screening to define the cancer immunome.
Keywords: genetics; lung non small cell cancer; carcinoma, non-small-cell lung; lung neoplasms; gene library; tumor antigen; lung tumor; blood; biosynthesis; immunology; dna; antigens, neoplasm; messenger rna; rna, messenger; dna, neoplasm; testis; humans; human; male; article; anion transport protein; anion transport proteins
Journal Title: Cancer Immunity
Volume: 4
ISSN: 1424-9634
Publisher: Academy of Cancer Immunology  
Date Published: 2004-11-17
Start Page: 13
End Page: 20
Language: English
PROVIDER: scopus
PUBMED: 15546177
Notes: Cancer Immun -- Cited By (since 1996):15 -- Export Date: 16 June 2014 -- Source: Scopus
MSK Authors
  1. Matthew J Scanlan
    48 Scanlan
  2. Sang Yull Lee
    16 Lee
  3. Andrew John Simpson
    31 Simpson
  4. Gerd Ritter
    158 Ritter
  5. Lloyd J Old
    385 Old
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