Breast cancer genome anatomy: Correlation of morphological changes in breast carcinomas with expression of the novel gene product Di12 Journal Article
| Authors: | Burger, A.; Li, H.; Zhang, X. K.; Pienkowska, M.; Venanzoni, M.; Vournakis, J.; Papas, T.; Seth, A. |
| Article Title: | Breast cancer genome anatomy: Correlation of morphological changes in breast carcinomas with expression of the novel gene product Di12 |
| Abstract: | To determine which genes may be activated or inactivated during breast cancer development, we employed two cloning strategies (subtractive hybridization and differential display) using RNA samples from a human breast tumor and its matching normal breast cell line. Of 950 clones isolated, 102 cDNA inserts mere analysed by DNA sequencing and database searching. We found 30 clones that were obviously unidentified, with no significant homology to any listed human gene. We focused upon one of the novel genes, Di12, that is differentially expressed as a 1.35 kb RNA in breast cancer tissues and cell-lines, and in several normal tissues. A full length cDNA of this gene was cloned, and its DNA sequence revealed an open reading frame of 339 amino acids. Antibodies to the ten N-terminal amino acids were developed to investigate the expression of Di12 in breast cancer cell-lines and tumors. The Di12 protein was found in tissue sections of infiltrating ductal carcinomas (IDCs), but not in benign or normal breast specimens. RT-PCR analysis confirmed expression of Di12 in 80% of infiltrating ductal carcinomas (IDCs). As IDC constitutes ~ 70% of breast cancers seen clinically, the level of Di12 expression may be predictive of disease progression. |
| Keywords: | immunohistochemistry; clinical article; controlled study; human tissue; human cell; sequence analysis; proteins; animals; reverse transcription polymerase chain reaction; breast cancer; gene expression; neoplasm proteins; gene product; tumor cells, cultured; breast neoplasms; rna; molecular cloning; cloning, molecular; oncogene; amino acid sequence; molecular sequence data; amino terminal sequence; rna, messenger; cancer infiltration; breast carcinoma; dna, neoplasm; protein biosynthesis; dna sequence; sequence homology; rna, neoplasm; antibody; complementary dna; carcinoma, ductal, breast; dna, complementary; open reading frame; rabbits; breast duct; humans; human; female; priority journal; article; subtractive cloning; molecular oncology; differential display; gene anatomy |
| Journal Title: | Oncogene |
| Volume: | 16 |
| Issue: | 3 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1998-01-22 |
| Start Page: | 327 |
| End Page: | 333 |
| Language: | English |
| PUBMED: | 9467958 |
| PROVIDER: | scopus |
| DOI: | 10.1038/sj.onc.1201517 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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