5'-Structural analysis of genes encoding polymorphic antigens of chemically induced tumors Journal Article
| Authors: | Srivastava, P. K.; Chen, Y. T.; Old, L. J. |
| Article Title: | 5'-Structural analysis of genes encoding polymorphic antigens of chemically induced tumors |
| Abstract: | We have proposed that the distinct tumor rejection antigens of chemically induced sarcomas in inbred mice belong to a family of M(r) 96,000 glycoproteins (gp96). An identical 14-amino acid sequence was found at the amino terminus of gp96 from two antigenically distinct BALB/c sarcomas. Oligonucleotide probes derived from this sequence permitted isolation of 5' cDNA and genomic fragments coding for gp96. Three short exons interrupted by relatively long introns were identified at the 5' terminus of the gp96 gene. The first exon encodes a signal peptide, which is consistent with gp96 being a cell surface antigen. Southern blot analysis indicated that the gp96 family is encoded by a single gene, and 3-kilobase transcripts were detected in all normal and tumor cells tested. Nucleotide and deduced amino acid sequences from 311 base pairs at the 5' terminus showed no homology with any known protein. The availability of molecular probes for the gp96 system permits analysis of the structural polymorphism of these antigens. |
| Keywords: | nonhuman; animal cell; mouse; heredity; animalia; sarcoma; membrane antigen; amino acid sequence; genetic engineering; structure analysis; tumor rejection; glycoprotein; priority journal; blood and hemopoietic system |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 84 |
| Issue: | 11 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1987-06-01 |
| Start Page: | 3807 |
| End Page: | 3811 |
| Language: | English |
| DOI: | 10.1073/pnas.84.11.3807 |
| PUBMED: | 2438686 |
| PROVIDER: | scopus |
| PMCID: | PMC304965 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work