Cloning and chromosome mapping of human retinoid X receptor β: Selective amino acid sequence conservation of a nuclear hormone receptor in mammals Journal Article
| Authors: | Fleischhauer, K.; McBride, O. W.; DiSanto, J. P.; Ozato, K.; Yang, S. Y. |
| Article Title: | Cloning and chromosome mapping of human retinoid X receptor β: Selective amino acid sequence conservation of a nuclear hormone receptor in mammals |
| Abstract: | The murine retinoid X receptor β (mRXRβ) is a nuclear hormone receptor that activates transcription of murine major histocompatibility complex (MHC) class I genes in response to retinoic acid. In this study, the human RXRβ gene was mapped onto the short arm or centromeric region of chromosome 6 (6pter-q13), which also harbors the MHC. Chromosomal localization was performed by Southern hybridization of genomic DNA from human rodent cell hybrids with the mRXRβ gene as a probe. In addition, a full-length cDNA clone encoding a human RXRβ was isolated by nucleic acid screening of a human cDNA library with a fragment of the mRXRβ gene as a probe. Comparison of the nucleotide-coding sequences of the human and the murine RXRβ revealed a predominance of third base substitutions, resulting in selective conservation of the predicted amino acid sequence of the proteins. The overall sequence homology was 97.6% on the amino acid level as opposed to 91.6% on the nucleotide level. In Northern hybridization experiments with the human cDNA as a probe, RXRβ gene transcripts were detected in a variety of human tumor cell lines, regardless of whether these cell lines expressed MHC class I genes. © 1993 Springer-Verlag. |
| Keywords: | human cell; nonhuman; comparative study; antigen expression; animal cell; mouse; animal; mice; nuclear proteins; molecular cloning; cloning, molecular; gene mapping; amino acid sequence; conserved sequence; molecular sequence data; sequence homology, amino acid; messenger rna; sequence alignment; nucleotide sequence; tumor cell line; base sequence; chromosomes, human, pair 6; sequence homology; sequence homology, nucleic acid; mammals; blotting, northern; genetic conservation; complementary dna; centromere; chromosomal localization; chromosome mapping; major histocompatibility antigen class 1; receptors, cell surface; northern blotting; sequence analysis, dna; southern blotting; nucleic acid base substitution; hybrid cell; hybrid cells; dna library; chromosome 6p; dna probes; human; priority journal; article; hamsters; retinoid binding protein |
| Journal Title: | Human Genetics |
| Volume: | 90 |
| Issue: | 5 |
| ISSN: | 0340-6717 |
| Publisher: | Springer |
| Date Published: | 1993-01-01 |
| Start Page: | 505 |
| End Page: | 510 |
| Language: | English |
| DOI: | 10.1007/bf00217449 |
| PUBMED: | 8381386 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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