Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene Journal Article
| Authors: | Mizuno, S.; Trapani, J. A.; Koller, B. H.; Dupont, B.; Yang, S. Y. |
| Article Title: | Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene |
| Abstract: | A cDNA clone, designated JTW15, has been isolated and found to encode a novel, divergent class I HLA gene transcript. The clone was identified by its ability to hybridize with a pan-HLA class I nucleotide probe while failing to bind probes specific for the 3' untranslated exons of the HLA-A, -B, and -C genes. JTW15, 1665 nucleotides in length, represents a full length copy of a ~1.7-kb mRNA detected in Northern blot analyses. Comparison of the derived amino acid sequence with those of other HLA class I proteins showed JTW15 to be the most divergent HLA class I gene identified so far, the level of sequence homology being lowest for the α1 and α2 domains. The JTW15 gene transcript was found in a broad variety of cell types and was inducible in cells treated with IFN-γ. JTW15 is the first isolated full length cDNA clone defining a non-HLA-ABC class I gene. |
| Keywords: | human cell; cell line; molecular cloning; cloning, molecular; dna; amino acid sequence; molecular sequence data; genetic engineering; cell culture; nucleotide sequence; hla-c antigens; base sequence; hla-a antigens; hla-b antigens; hla antigens; major histocompatibility antigen class 1; interferon type ii; genes, mhc class i; lymphoma, large-cell; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 140 |
| Issue: | 11 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-06-01 |
| Start Page: | 4024 |
| End Page: | 4030 |
| Language: | English |
| PUBMED: | 3131426 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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