Pathogen-specific CD8 T cell responses are directly inhibited by IL-10 Journal Article

Authors: Biswas, P. S.; Pedicord, V.; Ploss, A.; Menet, E.; Leiner, I.; Pamer, E. G.
Article Title: Pathogen-specific CD8 T cell responses are directly inhibited by IL-10
Abstract: Regulation of CD8 T cell expansion and contraction is essential for successful immune defense against intracellular pathogens. IL-10 is a regulatory cytokine that can restrict T cell responses by inhibiting APC functions. IL-10, however, can also have direct effects on T cells. Although blockade or genetic deletion of IL-10 enhances T cell-mediated resistance to infections, the extent to which IL-10 limits in vivo APC function or T cell activation/proliferation remains unknown. Herein, we demonstrate that primary and memory CD8 T cell responses following Listeria monocytogenes infection are enhanced by the absence of IL-10. Surface expression of the IL-10R is transiently up-regulated on CD8 T cells following activation, suggesting that activated T cells can respond to IL-10 directly. Consistent with this notion, CD8 T cells lacking IL-10R2 underwent greater expansion than wild-type T cells upon L. monocytogenes infection. The absence of IL-10R2 on APCs, in contrast, did not enhance T cell responses following infection. Our studies demonstrate that IL-10 produced during bacterial infection directly limits expansion of pathogen-specific CD8 T cells and reveal an extrinsic regulatory mechanism that modulates the magnitude of memory T cell responses. Copyright © 2007 by The American Association of Immunologists, Inc.
Keywords: signal transduction; controlled study; protein expression; nonhuman; flow cytometry; cd8+ t lymphocyte; cell proliferation; cd8-positive t-lymphocytes; animal cell; mouse; animals; mice; mice, knockout; cells, cultured; drug inhibition; interleukin 10; animal experiment; animal model; enzyme linked immunosorbent assay; lymphocyte activation; dendritic cells; upregulation; immunologic memory; listeriosis; listeria monocytogenes; interleukin-10; memory t lymphocyte; lymphocyte subpopulation; interleukin 10 receptor; receptors, interleukin-10
Journal Title: Journal of Immunology
Volume: 179
Issue: 7
ISSN: 0022-1767
Publisher: The American Association of Immunologists, Inc  
Date Published: 2007-10-01
Start Page: 4520
End Page: 4528
Language: English
PUBMED: 17878348
PROVIDER: scopus
Notes: --- - "Cited By (since 1996): 16" - "Export Date: 17 November 2011" - "CODEN: JOIMA" - "Source: Scopus"