Differential in vitro activation of CD8(-)CD4(+) and CD4(-)CD8(+) T lymphocytes by combinations of anti-CD2 and anti-CD3 antibodies Journal Article
| Authors: | Yang, S. Y.; Rhee, S.; Welte, K.; Dupont, B. |
| Article Title: | Differential in vitro activation of CD8(-)CD4(+) and CD4(-)CD8(+) T lymphocytes by combinations of anti-CD2 and anti-CD3 antibodies |
| Abstract: | Purified peripheral blood T lymphocytes and the CD8-CD4+ and CD4-CD8+ T cell subsets, exhaustively depleted of APC have been studied for their capacity to respond to mAb directed against the CD3-Ti Ag-specific TCR complex and against the CD2 SRBCR. It is demonstrated that high affinity IL-2R can be readily induced by either anti-CD3 and/or anti-CD2 stimulation. However, IL-2 production can be observed only in the CD4+CD8- T cell subset. These results clearly contrast data obtained with purified CF4-CD8+ T cells, which are able to proliferate when the CD3-Ti complex is activated in the presence of APC. The data presented in the present study demonstrate that a simplified model for T cell activation and clonal expansion of the two major T cell subsets involve only the CD3-Ti complex and the CD2 Ag. Under conditions where the activation signals for the T cells are restricted only to the activation of CD3-Ti and CD2, the CD4+CD8- T cells respond with IL-2 production and expression of high affinity IL-2, whereas the CD4-CD8+ T cell subset depends on exogenous IL-2 provided by the CD4+CD8- cells. These data do not, however, exclude an involvement of other cell-surface signals for regulation and control of T cell activation and T cell effector functions. |
| Keywords: | human cell; cd3 antigen; t-lymphocytes; phenotype; interleukin 2; monoclonal antibody; cd2 antigen; lymphocyte activation; antibodies, monoclonal; cell culture; receptors, antigen, t-cell; cell separation; t lymphocyte subpopulation; t lymphocyte activation; normal human; interleukin-2; antigens, differentiation, t-lymphocyte; dose-response relationship, immunologic; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; phytohemagglutinins |
| Journal Title: | Journal of Immunology |
| Volume: | 140 |
| Issue: | 7 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-04-01 |
| Start Page: | 2115 |
| End Page: | 2120 |
| Language: | English |
| PUBMED: | 3258328 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
Citation Impact
Related MSK Work