Differential sensitivity of CD4(+) and CD8(+) T lymphocytes to phorbol myristate acetate upon Anti-CD3 stimulation: Evidence for a distinct signaling pathway Journal Article
| Authors: | Chouaib, S.; Mahe, Y.; Mechri, S.; Andreeff, M.; Welte, K. |
| Article Title: | Differential sensitivity of CD4(+) and CD8(+) T lymphocytes to phorbol myristate acetate upon Anti-CD3 stimulation: Evidence for a distinct signaling pathway |
| Abstract: | The effects of suboptimal concentrations of Phorbol Myristate Acetate (PMA) in combination with submitogenic concentrations of OKT3 antibody on CD4+ and CD8+ cell activation were investigated. Upon stimulation with OKT3 (25 pg/ml) and PMA (0.25 - 0.75 ng/ml), the majority of CD4+ cells entered the cell cycle whereas most of CD8+ cells remained in G0/G1 phase. Under the same conditions of OKT3 stimulations, both CD4+ and CD8+ cells failed to produce IL2 in the absence of PMA. In the presence of PMA (0,25 ng/ml), CD4+ produced measurable amounts of IL2 (0,5 - 2,3 U/ml) whereas IL2 production by CD8+ cells remained below the detection limit. Expression of TAC antigen (CD25) was found to parallel IL2 production and cell proliferation in both subsets whereas changes in [Ca2+] mobilization following OKT3 stimulation were similar in both subsets. Interestingly, the elevation of intracellular cyclic adenosine 3':5' monophosphate (cAMP) was not equally distributed between CD4+ and CD8+ subpopulations. Furthermore, the kinetics of protein kinase (PKC) translocation was markedly prolonged in membranes of CD4+ cells compared with CD8+ cells suggesting a differential involvement that may operate under distinct regulatory signaling mechanisms. |
| Keywords: | cell activation; interleukin-2; expression; activation; inhibition; monoclonal-antibody; molecules; cyclic-amp; cell growth-factor; protein kinase-c; t-cells subsets; pkc translocation; cytoplasmic free calcium |
| Journal Title: | International Journal of Immunopathology and Pharmacology |
| Volume: | 4 |
| Issue: | 1 |
| ISSN: | 0394-6320 |
| Publisher: | Biomedical Research Press, Sas |
| Date Published: | 1991-01-01 |
| Start Page: | 19 |
| End Page: | 32 |
| Language: | English |
| ACCESSION: | WOS:A1991GJ56200003 |
| DOI: | 10.1177/039463209100400103 |
| PROVIDER: | wos |
| Notes: | Source: Wos |
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