A sequence element associated with the Plasmodium falciparum KAHRP gene is the site of developmentally regulated protein-DNA interactions Journal Article

   


Authors: Lanzer, M.; de Bruin, D.; Ravetch, J. V.
Article Title: A sequence element associated with the Plasmodium falciparum KAHRP gene is the site of developmentally regulated protein-DNA interactions
Abstract: The Plasmodium falciparum gene encoding the knob associated histidine-rich protein (KAHRP) is shown to be transcriptionally regulated during its expression in the intraerythrocytic cycle as demonstrated by stage specific nuclear run-on analysis. The genomic organization of the KAHRP gene was determined and the structural basis for the stage specific transcription investigated. A sequence motif with two-fold symmetry was found 160 bp upstream of the RNA initiation site. This sequence element interacts with parasite derived nuclear extracts in a stage specific manner that correlates with the transcriptional activity of the KAHRP gene. These studies suggest a functional role for this structural element in the developmental regulation of a P. falciparum erythrocytic gene. © 1992 IRL Press at Oxford University Press.
Keywords: nonhuman; animal; gene; transcription initiation; transcription, genetic; gene expression regulation; molecular sequence data; peptides; base sequence; development; erythrocyte; plasmodium falciparum; dna responsive element; blotting, northern; gene structure; protein dna interaction; protozoan proteins; host parasite interaction; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, non-p.h.s.
Journal Title: Nucleic Acids Research
Volume: 20
Issue: 12
ISSN: 0305-1048
Publisher: Oxford University Press  
Date Published: 1992-06-25
Start Page: 3051
End Page: 3056
Language: English
DOI: 10.1093/nar/20.12.3051
PUBMED: 1620601
PROVIDER: scopus
PMCID: PMC312436
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026683042&doi=10.1093%2fnar%2f20.12.3051&partnerID=40&md5=7ae7e04891592e3ca49c31b29ca7d55c
Notes: Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Jeffrey V. Ravetch
    72 Ravetch
  2. Derik de Bruin
    13 de Bruin
Related MSK Work