Transcriptional and nucleosomal characterization of a subtelomeric gene cluster flanking a site of chromosomal rearrangements in Plasmodium falciparum Journal Article

   


Authors: Lanzer, M.; de Bruin, D.; Wertheimer, S. P.; Ravetch, J. V.
Article Title: Transcriptional and nucleosomal characterization of a subtelomeric gene cluster flanking a site of chromosomal rearrangements in Plasmodium falciparum
Abstract: falciparum chromosomes are compartmentalized into different domains: conserved and polymorphic domains; transcriptionally active and silent domains. Here we have analyzed the transition between these domains at the structural and nucleosomal level. This study was conducted with the end of chromosome 2 that is associated with cytoadherence. At this end of the chromosome, the first set of erythrocytic genes has been mapped 85 kb from the telomere. These genes are monocistronically transcribed as revealed by nuclear run-on analysis. Two of these genes, P1EMP3 and KAHRP, are deleted in cytoadherent negative mutants, whereas the third gene, GLARP, is expressed in all the strains investigated. The data indicate that the polymorphic domain at this end of chromosome 2 extends into the transcribed region. Analysis of the chromatin structure revealed that both the transcribed domain and the subtelomeric region are organized as nucleosomes with a periodicity of 155 ± 5 bp. © 1994 Oxford University Press.
Keywords: controlled study; gene cluster; gene deletion; mutation; nonhuman; chromosome structure; telomere; animal; genetic transcription; transcription, genetic; rna, messenger; chromatin; chromosome rearrangement; plasmodium falciparum; cell adhesion; chromosome 2; deletion mutant; chromosome mapping; multigene family; chromatin structure; nucleosome; nucleosomes; rna probes; priority journal; article; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, non-p.h.s.; dna, protozoan; chromosomes, artificial, yeast; chromosome polymorphism; genes, protozoan
Journal Title: Nucleic Acids Research
Volume: 22
Issue: 20
ISSN: 0305-1048
Publisher: Oxford University Press  
Date Published: 1994-10-11
Start Page: 4176
End Page: 4182
Language: English
DOI: 10.1093/nar/22.20.4176
PROVIDER: scopus
PMCID: PMC331913
PUBMED: 7937144
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028029839&doi=10.1093%2fnar%2f22.20.4176&partnerID=40&md5=c87aa2ceb3c7b8a2ad60401bbc4541c2
Notes: Source: Scopus
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MSK Authors
  1. Jeffrey V. Ravetch
    72 Ravetch
  2. Derik de Bruin
    13 de Bruin
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