Transcriptional differences in polymorphic and conserved domains of a complete cloned P. falciparum chromosome Journal Article
| Authors: | Lanzer, M.; De Bruin, D.; Ravetch, J. V. |
| Article Title: | Transcriptional differences in polymorphic and conserved domains of a complete cloned P. falciparum chromosome |
| Abstract: | CLASSICAL genetic studies on the human malaria parasite Plasmodium falciparum have been hampered by a complex life cycle which alternates between vertebrate and invertebrate hosts. Consequently, only a few genetic crosses have been performed so far1-4. In addition, molecular genetics has provided only limited access to the genes of this pathogen, a consequence of an unusually high A + T content5,6. To overcome these limitations we have constructed an ordered telomere-to-telomere contig map of P. falciparum chromosome 2 by isolating overlapping yeast artificial chromosome clones. This approach was used to examine the strain-dependent polymorphisms commonly observed for P. falciparum chromosomes7,8. Our analysis reveals that polymorphisms of chromosome 2 are restricted to regions at either end, representing 20% of the chromosome. Transcription mapping of the entire chromosome suggests a compartmentalization of chromosome 2 into a transcribed central domain and silent polymorphic ends. © 1993 Nature Publishing Group. |
| Keywords: | nonhuman; chromosome; animal; genetic transcription; transcription, genetic; gene library; vertebrata; molecular cloning; cloning, molecular; gene mapping; molecular sequence data; base sequence; nucleic acid hybridization; plasmodium falciparum; chromosome mapping; chromosomes, fungal; invertebrata; deoxyribonucleases, type ii site-specific; priority journal; article; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, non-p.h.s.; dna, protozoan; genes, protozoan |
| Journal Title: | Nature |
| Volume: | 361 |
| Issue: | 6413 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1993-02-18 |
| Start Page: | 654 |
| End Page: | 657 |
| Language: | English |
| DOI: | 10.1038/361654a0 |
| PUBMED: | 8437626 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
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