A YAC contig map of Plasmodium falciparum chromosome 4: Characterization of a DNA amplification between two recently separated isolates Journal Article

  


Authors: Rubio, J. P.; Triglia, T.; Kemp, D. J.; de Bruin, D.; Ravetch, J. V.; Cowman, A. F.
Article Title: A YAC contig map of Plasmodium falciparum chromosome 4: Characterization of a DNA amplification between two recently separated isolates
Abstract: We have generated a physical map of Plasmodium falciparum chromosome 4 using yeast artificial chromosomes (YACs). The map is defined by a YAC contig spanning approximately 1.05 Mb, which has been restriction mapped to a resolution of 30 kb and is punctuated by 22 sequence-tagged sites. The physical information obtained has enabled us to compare and contrast the structure of chromosome 4 in detail between FCR3 and B8, two recently separated isolates of P. falciparum, leading to characterization of a novel chromosome polymorphism occurring in a subtelomeric region. Comparison of chromosomes 4 from 10 different isolates has shown that chromosome size polymorphisms are restricted to both subtelomeric regions. These analyses provide a high-resolution physical map that will be important to complement genetic analysis of this human pathogen. © 1995.
Keywords: nonhuman; comparative study; genetic analysis; polymerase chain reaction; animal; gene amplification; molecular sequence data; base sequence; genome; yeast; plasmodium falciparum; chromosome map; chromosome mapping; chromosome 4; gene isolation; priority journal; article; support, non-u.s. gov't; chromosomes, artificial, yeast; sequence tagged sites; genes, protozoan
Journal Title: Genomics
Volume: 26
Issue: 2
ISSN: 0888-7543
Publisher: Elsevier Inc.  
Date Published: 1995-03-20
Start Page: 192
End Page: 198
Language: English
DOI: 10.1016/0888-7543(95)80200-6
PUBMED: 7601442
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028987722&doi=10.1016%2f0888-7543%2895%2980200-6&partnerID=40&md5=650b2c535f82951406a93817b1468287
Notes: Source: Scopus
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MSK Authors
  1. Jeffrey V. Ravetch
    72 Ravetch
  2. Derik de Bruin
    13 de Bruin
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