| Abstract: |
Cytogenetic and molecular genetic analyses have shown that the 12q22 region is recurrently deleted in male germ cell tumors (GCTs), suggesting that this site may harbor a tumor suppressor gene (TSG). Previous loss of heterozygosity (LOH) analyses identified a consensus minimal deleted region between the markers D12S377 and D12S296, and a YAC clone contig covering the region was generated. Here, we describe a high-resolution sequence-ready physical map of this contig covering a 3-Mb region. The map comprised of 52 cosmids, 49 PACs, and 168 BACs that were anchored to the previous YAC contig; 99 polymorphic, nonpolymorphic, EST, and gene-based markers are now placed on this map in a unique order. Of these, 61 markers were isolated in the present study, including one that was polymorphic. In addition, we have narrowed the minimal deletion to ~ 830 kb between D12S1716 (proximal) and P382A8-AG (distal) by LOH analysis of 108 normal-tumor DNAs from GCT patients using 21 polymorphic STSs. These physical and deletion maps should prove useful for identification of the candidate TSG in GCTs, provide framework to generate complete DNA sequence, and ultimately generate a gene map of this segment of the chromosome 12. |
| Keywords: |
controlled study; human tissue; major clinical study; gene deletion; sequence deletion; genes; cloning, molecular; gene mapping; molecular sequence data; nucleotide sequence; dna, neoplasm; testicular neoplasms; dna sequence; germ cell tumor; expressed sequence tags; genetic marker; chromosome 12q; genetic markers; chromosomes, bacterial; sequence analysis, dna; cell dna; consensus sequence; germinoma; chromosomes, human, pair 12; humans; human; male; priority journal; article; cosmid; contig; contig mapping; physical chromosome mapping; sequence tagged sites; bacteriophage p1
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