Transcription mapping of a 100 kb locus of Plasmodium falciparum identifies an intergenic region in which transcription terminates and reinitiates Journal Article
| Authors: | Lanzer, M.; de Bruin, D.; Ravetch, J. V. |
| Article Title: | Transcription mapping of a 100 kb locus of Plasmodium falciparum identifies an intergenic region in which transcription terminates and reinitiates |
| Abstract: | We have mapped Plasmodium falciparum erythrocytic stage transcription units on chromosome 10 in the vicinity of the gene encoding the glycophorin binding protein (GBP130) using yeast artificial chromosomes (YACs). Three erythrocytic stage transcription units are clustered in a 40 kb region. Two of these genes are closely linked, separated by <2 kb. Nuclear run-on data demonstrate that transcription of these two genes, though unidirectional, is monocistronic. Within this intergenic region are the sites at which transcription of the upstream gene terminates and the GBP130 gene initiates. These studies represent the first description of the minimal and necessary cis-acting elements for transcription termination and initiation in this protozoan parasite. |
| Keywords: | promoter region; nonhuman; animal; gene expression; genetic transcription; transcription, genetic; gene library; rna; gene mapping; dna; amino acid sequence; molecular sequence data; base sequence; binding protein; gene induction; plasmodium falciparum; rna processing; blotting, northern; protozoa; genome, human; genetic linkage; chromosomes, fungal; promoter; transcription termination; chromosome 10; glycophorin; human; priority journal; article; electrophoresis, agar gel; support, non-u.s. gov't; support, u.s. gov't, non-p.h.s.; dna, protozoan; terminator regions (genetics); sv40 enhancer; yeast artificial chromosomes; malaria parasite |
| Journal Title: | EMBO Journal |
| Volume: | 11 |
| Issue: | 5 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1992-05-01 |
| Start Page: | 1949 |
| End Page: | 1955 |
| Language: | English |
| PUBMED: | 1374714 |
| PROVIDER: | scopus |
| PMCID: | PMC556654 |
| DOI: | 10.1002/j.1460-2075.1992.tb05248.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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