A co-stimulatory role for CD28 in the activation of CD4(+) t lymphocytes by staphylococcal enterotoxin B Journal Article

Authors: Goldbach-Mansky, R.; King, P. D.; Taylor, A. P.; Dupont, B.
Article Title: A co-stimulatory role for CD28 in the activation of CD4(+) t lymphocytes by staphylococcal enterotoxin B
Abstract: In this study we investigated the differential effect of the co-stimulatory receptor ligand molecules CD2/LFA-3, LFA-1/ICAM-1, and CD28/B7 on microblal superantigen mediated activation of CD4+ T cells. Highly purified CD4+ T cells, depleted of antigen presenting cells (APCs), do not proliferate in response to the superantigen, staphyiococcal enterotoxin B (SEB). However, CD4+ T cells do respond to SEB in the presence of the LFA-3, ICAM-1, and B7 positive erythroleukemic cell line K562, murine L cells, human B7 transfected L cells or CD28 mAb. The K562 plus SEB induced response can be inhibited by combinaction of mAbs to CD2 and LFA-1, and to LFA-3, ICAM-1, and B7. Addition of CD28 mAb to the CD2 and LFA-1 Inhibited cultures could restore the response. Furthermore, soluble CD28 mAb alone is able to synergize with SEB to induce a proliferative CD4+ T cell response. CD4+ T cells depleted of APCs could also be activated by a pool of four mAbs directed to the Vβ5, Vβ6, Vβ8, and Vβ12 region of the TCR when a co-stimulatory signal was provided by the CD28 mAb, while the Vβ alone or in combination are unable to activate CD4+ T cells in the absence of APCs. In contrast, addition of soluble mAbs to CD2 and LFA-1 molecules failed to co-stimulate SEB activated CD4+ T lymphocytes. The kinetics of the different modes of activation are distinct. SEB induced proliferation is most efficient in the presence of autoiogous APCs with maximal proliferation at a log4 lower SEB concentration than when CD28 mAbs were used. SEB plus K562 activation peaks on day 7, while SEB plus CD28 mAb induced proliferative responses do not peak until day 9. Thus, superantigen mediated activation of CD4+ T cells requires co-stimulatory signals, among which CD28 has distinct and unique effects. © 1992 Oxford University Press.
Keywords: signal transduction; adult; human cell; t lymphocyte; animal; mice; cells, cultured; tumor cells, cultured; transfection; monoclonal antibody; antigen presentation; lymphocyte activation; antibodies, monoclonal; cd4-positive t-lymphocytes; cd4 antigen; antigens, cd; staphylococcus aureus; antigen presenting cell; cd28 antigen; antigens, cd28; receptors, antigen, t-cell, alpha-beta; normal human; antigens, differentiation, t-lymphocyte; leukemia, erythroblastic, acute; superantigen; enterotoxins; staphylococcus enterotoxin b; human; priority journal; article; support, u.s. gov't, p.h.s.; l cells (cell line); anti-cd28; cd4+ antigen
Journal Title: International Immunology
Volume: 4
Issue: 12
ISSN: 0953-8178
Publisher: Oxford University Press  
Date Published: 1992-12-01
Start Page: 1351
End Page: 1360
Language: English
DOI: 10.1093/intimm/4.12.1351
PUBMED: 1363054
PROVIDER: scopus
Notes: Article -- Export Date: 30 July 2019 -- Source: Scopus
Citation Impact
MSK Authors
  1. Bo Dupont
    241 Dupont
  2. Philip D. King
    19 King