ICAM-1 (CD54) expression on B lymphocytes is associated with their costimulatory function and can be increased by coactivation with IL-1 and IL-7 Journal Article

Authors: Dennig, D.; Lacerda, J.; Yan, Y.; Gasparetto, C.; O'Reilly, R. J.
Article Title: ICAM-1 (CD54) expression on B lymphocytes is associated with their costimulatory function and can be increased by coactivation with IL-1 and IL-7
Abstract: Recent studies have demonstrated that acute lymphoblastic leukemia-derived pre-B cell lines are deficient in their costimulatory function for T cell proliferation in response to the mitogen Con A and the superantigens TSST-1 and SEB. Stimulation of these pre-B cells with IL-7 increased their costimulatory function which involved the B7/CD28 pathway. In the present study, we stimulated T cells with Con A, TSST-1, and SEB in the presence of peripheral blood B lineage cells that do not constitutively express B7/BB1 on their surface and investigated whether their costimulatory function could also be enhanced by IL-7. We found that, in the presence of IL-1, stimulation with IL-7 increased the costimulatory function of B cells and their surface expression level of ICAM-1 (CD54). We then investigated whether costimulatory B cell function could be inhibited by blocking the ICAM-1/LFA-1 pathway. Addition of anti-ICAM-1 mAb to the coculture of T and B cells inhibited T cell proliferation by approximately 20%. In contrast, addition of anti-LFA-1β (CD18) mAb, directed against the T cell ligand of ICAM-1, inhibited T cell proliferation almost completely. To determine the role of ICAM-1 in costimulatory B cell function, we sorted B cells into ICAM-1low-and ICAM-1high-expressing populations. We found that B cells expressing high levels of surface ICAM-1 elicited significantly higher T cell responses than those with low levels, suggesting that the expression level of ICAM-1 on peripheral blood B cells correlates with their costimulatory function. © 1994 Academic Press Inc.
Keywords: human cell; antigen expression; cell proliferation; t-lymphocytes; cell function; interleukin 7; cell population; acute lymphoblastic leukemia; b-lymphocytes; t lymphocyte receptor; lymphocyte activation; antibodies, monoclonal; cell adhesion molecules; cell stimulation; cell interaction; intercellular adhesion molecule 1; in vitro; interleukin-7; intercellular adhesion molecule-1; lymphocyte function-associated antigen-1; interleukin 1; interleukin-1; lymphocyte function associated antigen 1; b lymphocyte activation; dose-response relationship, immunologic; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; lymphocyte cooperation
Journal Title: Cellular Immunology
Volume: 156
Issue: 2
ISSN: 0008-8749
Publisher: Elsevier Inc.  
Date Published: 1994-07-01
Start Page: 414
End Page: 423
Language: English
DOI: 10.1006/cimm.1994.1186
PROVIDER: scopus c2 - 7912996
PUBMED: 7912996
Notes: Export Date: 14 January 2019 -- Article -- Source: Scopus
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MSK Authors
  1. Richard O'Reilly
    519 O'Reilly
  2. Ying   Yan
    13 Yan