The B7/BB1 antigen provides one of several costimulatory signals for the activation of CD4+ T lymphocytes by human blood dendritic cells in vitro Journal Article
| Authors: | Young, J. W.; Koulova, L.; Soergel, S. A.; Clark, E. A.; Steinman, R. M.; Dupont, B. |
| Article Title: | The B7/BB1 antigen provides one of several costimulatory signals for the activation of CD4+ T lymphocytes by human blood dendritic cells in vitro |
| Abstract: | T cells respond to peptide antigen in association with MHC products on antigen-presenting cells (APCs). A number of accessory or costimulatory molecules have been identified that also contribute to T cell activation. Several of the known accessory molecules are expressed by freshly isolated dendritic cells, a distinctive leukocyte that is the most potent APC for the initiation of primary T cell responses. These include ICAM-1 (CD54), LFA-3 (CD58), and class I and II MHC products. Dendritic cells also constitutively express the accessory ligand for CD28, B7/BB1, which has not been previously identified on circulating leukocytes freshly isolated from peripheral blood. Dendritic cell expression of both B7/BB1 and ICAM-1 (CD54) increases after binding to allogeneic T cells. Individual mAbs against several of the respective accessory T cell receptors, e.g., anti-CD2, anti-CD4, anti-CD11a, and anti-CD28, inhibit T cell proliferation in the dendritic cell-stimulated allogeneic mixed leukocyte reaction (MLR) by 40-70%. Combinations of these mAbs are synergistic in achieving near total inhibition. Other T cell-reactive mAbs, e.g., anti-CD5 and anti-CD45, are not inhibitory. Lymphokine secretion and blast transformation are similarly reduced when active accessory ligand-receptor interactions are blocked in the dendritic cell-stimulated allogeneic MLR. Dendritic cells are unusual in their comparably higher expression of accessory ligands, among which B7/BB1 can now be included. These are pertinent to the efficiency with which dendritic cells in small numbers elicit strong primary T cell proliferate and effector responses. |
| Keywords: | human cell; cells, cultured; cell growth; dendritic cell; monoclonal antibody; t lymphocyte receptor; lymphocyte activation; dendritic cells; lymphocyte culture test, mixed; immune response; antigen; major histocompatibility antigen class 2; cd4-positive t-lymphocytes; ligand; cell adhesion molecules; effector cell; cd4 antigen; antigens, cd; major histocompatibility complex; isoantigens; leukocyte; intercellular adhesion molecule 1; lymphocyte function associated antigen 3; antigen presenting cell; antigen-presenting cells; t lymphocyte activation; cd28 antigen; antigens, cd28; mixed lymphocyte reaction; lymphoblastoid cell line; interleukin-2; antigens, surface; intercellular adhesion molecule-1; receptors, immunologic; major histocompatibility antigen class 1; cd28; costimulation; receptor upregulation; antigens, cd80; antigens, differentiation, t-lymphocyte; t cell activation; antigens, cd2; human; male; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; lymphokine; b7/bb1 |
| Journal Title: | Journal of Clinical Investigation |
| Volume: | 90 |
| Issue: | 1 |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Date Published: | 1992-07-01 |
| Start Page: | 229 |
| End Page: | 237 |
| Language: | English |
| DOI: | 10.1172/jci115840 |
| PUBMED: | 1378854 |
| PROVIDER: | scopus |
| PMCID: | PMC443085 |
| DOI/URL: | |
| Notes: | Lidia Koulova's name is misspelled on the original publication -- Correction issued, see DOI: 10.1172/JCI115840C1 -- Article -- Source: Scopus |
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