IL-7 induces surface expression of B7/BB1 on pre-B cells and an associated increase in their costimulatory effects on T cell proliferation Journal Article
| Authors: | Dennig, D.; O'Reilly, R. J. |
| Article Title: | IL-7 induces surface expression of B7/BB1 on pre-B cells and an associated increase in their costimulatory effects on T cell proliferation |
| Abstract: | Treatment with IL-7 of NALM-6 pre-B cells, but not of EBV-LCL, Daudi, HeLa, or K562 cells resulted in enhanced costimulatory activity for TSST-1- induced T cell proliferation. The effect of IL-7 on the costimulatory function of NALM-6 cells was dose-dependent, could be inhibited by a neutralizing anti-IL-7 mAb, and resulted in the need of less costimulatory cells, or less superantigen, to obtain proper T cell proliferation. Addition of anti-IL-7 mAb to the coculture of superantigen-stimulated T cells with IL- 7-pretreated NALM-6 cells did not alter the T cell responses obtained without addition of this antibody. These findings suggest that a possible costimulation of T cells by surface-bound IL-7 on pretreated NALM-6 cells did not contribute to the enhanced costimulatory function observed. Rather, these studies implicate IL-7-induced alterations of the NALM-6 cells themselves as the basis for the enhanced costimulatory activity observed. The most remarkable phenotypic differences between efficient costimulatory B lineage cells and deficient pre-B cells are a lower expression of ICAM-1 (CD54) and a lack of expression of the costimulatory B cell activation antigen B7/BB1. Upon activation with IL-7, the expression of ICAM-1 was increased, and the expression of B7/BB1 antigens was induced. The increased T cell responses in the presence of IL-7-treated NALM-6 cells could be inhibited by addition of anti-BB1 mAb. These results suggest that B7/BB1-negative pre-B cells may acquire the surface expression of B7/BB1 upon stimulation with IL-7, a process which is associated with increased costimulatory function for T cell proliferation. © 1994 Academic Press. All rights reserved. |
| Journal Title: | Cellular Immunology |
| Volume: | 153 |
| Issue: | 1 |
| ISSN: | 0008-8749 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1994-01-01 |
| Start Page: | 227 |
| End Page: | 238 |
| Language: | English |
| DOI: | 10.1006/cimm.1994.1020 |
| PROVIDER: | scopus |
| PUBMED: | 7507002 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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