Dissection of the function of HLA class II and costimulation in B cell- mediated and toxic shock syndrome toxin-1-induced T cell proliferation Journal Article
| Authors: | Dennig, D.; O'Reilly, R. J. |
| Article Title: | Dissection of the function of HLA class II and costimulation in B cell- mediated and toxic shock syndrome toxin-1-induced T cell proliferation |
| Abstract: | In this study we have demonstrated that costimulation for TSST-1-induced T cell proliferation is provided by HLA class II negative accessory B cells. In addition, TSST-1 binding to these HLA class II negative accessory B cells could not be detected by immunocytofluorescence, or in a system in which T cell proliferation depended on TSST-1 pretreated HLA class II negative B cells. These findings suggest that accessory signals can be supplied by B cells that are not involved in the activation of T cells via Ag-HLA/TCR-CD3 complex interaction: TCR ligation by a TSST-1/HLA complex and costimulation need not be delivered by the same cell. In the presence of HLA class II negative B cells, T cell proliferation in response to TSST-1 could be achieved despite addition of a mAb that can block HLA class II binding sites for TSST-1. These findings suggest the possibility that TSST-1 may directly ligate TCR. In the presence of anti-BB1-treated HLA class II negative accessory cells, TSST-1-induced T cell proliferation was inhibited by over 50% indicating the involvement of the BB1/CD28 pathway. The leukemic pre-B cell lines NALM-6, and BLIN-1, both of which express surface HLA class II Ag, but not the costimulatory B cell activation Ag BB1, were deficient in their accessory function for T cell proliferation in response to the superantigen TSST-1 and the mitogen Con A. |
| Keywords: | controlled study; human cell; cell proliferation; t-lymphocytes; b lymphocyte; b-lymphocytes; lymphocyte activation; antibodies, monoclonal; hla antigen class 2; histocompatibility antigens class i; hematopoietic stem cells; staphylococcus aureus; histocompatibility antigens class ii; t lymphocyte activation; pre b lymphocyte; antigens, surface; hla system; antigens, cd80; antigens, bacterial; enterotoxins; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; toxic shock syndrome |
| Journal Title: | Journal of Immunology |
| Volume: | 150 |
| Issue: | 12 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1993-06-15 |
| Start Page: | 5231 |
| End Page: | 5240 |
| Language: | English |
| PUBMED: | 7685791 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
Citation Impact
Related MSK Work