A novel HLA class II-independent TCR-mediated T cell activation mechanism is distinguished by the Vβ specificity of the proliferating oligoclones and their capacity to generate interleukin-2 Journal Article


Authors: Dennig, D.; Yan, Y.; Ferguson, K.; O'Reilly, R. J.
Article Title: A novel HLA class II-independent TCR-mediated T cell activation mechanism is distinguished by the Vβ specificity of the proliferating oligoclones and their capacity to generate interleukin-2
Abstract: Superantigens can induce proliferative T cell responses after complex formation with major histocompatibility complex (MHC) class II antigens. In this study, highly purified variant T cells from a histocompatibility leukocyte antigen (HLA) (human major histocompatibility complex) class II-deficient patient were stimulated with toxic shock syndrome toxin 1 (TSST-1) in the presence of either HLA class II-negative or normal antigen-presenting cells (APC). The proliferative responses were similar to those of normal T cells even when HLA class II structures were absent on both responding T cells and costimulatory APC. However, the Vβ specificity of responding T cells differed depending on the presence or absence of HLA class II antigens on the APC. In the presence of HLA class II-negative costimulatory APC, TSST-1 induced primarily proliferation of Vβ2-expressing T cells, whereas in the presence of normal APC virtually all responding T cells expressed Vβ elements different from Vβ2. In addition, in the presence of normal APC, T cells responding to TSST-1 produced much higher amounts of interleukin-2 than T cells proliferating in the presence of HLA class II-negative APC. These findings suggest that T cells bearing selected Vβ elements can directly interact with and respond to superantigen without involvement of HLA class II structures. Thus, our findings introduce a novel and distinct T cell receptor-mediated activation mechanism by which specific subpopulations of T cells respond to superantigen in the absence of HLA class II structures on APC.
Keywords: controlled study; unclassified drug; human cell; lymphocyte proliferation; interleukin 2; t lymphocyte receptor; hla antigen class 2; antigen presenting cell; t lymphocyte activation; superantigen; human; priority journal; article; staphylococcus toxin; toxic shock syndrome toxin 1
Journal Title: Cellular Immunology
Volume: 171
Issue: 2
ISSN: 0008-8749
Publisher: Elsevier Inc.  
Date Published: 1996-08-01
Start Page: 200
End Page: 210
Language: English
DOI: 10.1006/cimm.1996.0194
PUBMED: 8806788
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Richard O'Reilly
    519 O'Reilly
  2. Ying   Yan
    13 Yan