Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol Journal Article
| Authors: | Orci, L.; Palmer, D. J.; Amherdt, M.; Rothman, J. E. |
| Article Title: | Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol |
| Abstract: | TRANSPORT vesicles derived from the Golgi apparatus are thought to mediate biosynthetic transport across the Golgi stack1-3. These vesicles are surrounded by a protein coat4 whose principal constituents are coatomer (a complex of seven distinct subunits or COPs)5 and ADP-ribosylation factor (ARF, an N-myristylated small GTP-binding protein). The coat proteins of the COP-coated vesicles were originally defined by ultrastructural criteria4-9, however, and it is possible that important but minor coat proteins or cytoplasmic proteins needed for coat assembly may have been overlooked. Here we show that coatomer and ARF are the only cytoplasmic proteins needed for the assembly and budding of COP-coated vesicles. COP-coated buds may therefore form essentially by self-assembly from Golgi cisternae after an initial step in which GTP is used to allow ARF binding. © 1993 Nature Publishing Group. |
| Keywords: | nonhuman; proteins; animal; animal tissue; gtp-binding proteins; membrane proteins; liver; guanine nucleotide binding protein; cytosol; organelles; golgi complex; adenosine diphosphate ribosylation factor; clathrin; rabbits; coated vesicle; rabbit; golgi apparatus; adp-ribosylation factors; microscopy, immunoelectron; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; macromolecular systems |
| Journal Title: | Nature |
| Volume: | 364 |
| Issue: | 6439 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1993-08-19 |
| Start Page: | 732 |
| End Page: | 734 |
| Language: | English |
| DOI: | 10.1038/364732a0 |
| PUBMED: | 8355790 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
