Stepwise assembly of functionally active transport vesicles Journal Article
| Authors: | Ostermann, J.; Orci, L.; Tani, K.; Amherdt, M.; Ravazzola, M.; Elazar, Z.; Rothman, J. E. |
| Article Title: | Stepwise assembly of functionally active transport vesicles |
| Abstract: | Budding of COP-coated vesicles (the likely carriers of newly synthesized proteins from the endoplasmic reticulum through the Golgi stack) from Golgi cisternae requires ADP-ribosylation factor (ARF), coatomer proteins from the cytosol, GTP, and fatty acyl-coenzyme A (CoA). The assembly of coated buds on the membranes requires coatomer, ARF, and GTP. When palmitoyl-CoA is added, membrane fission occurs at the coated bud, releasing coated vesicles. We show that COP-coated vesicles can be generate stepwise in vitro and isolated in a functionally active form, demonstrating that the minimal set of cytosolic components required for their formation as well as principal steps in their assembly have been identified. © 1993. |
| Keywords: | nonhuman; animal cell; animal; gtp-binding proteins; membrane proteins; intracellular transport; morphogenesis; animalia; endoplasmic reticulum; membrane glycoproteins; protein transport; guanosine triphosphate; cytosol; in vitro; organelles; golgi complex; cho cells; adenosine diphosphate ribosylation factor; membrane vesicle; palmitoyl coenzyme a; microtubule-associated proteins; intracellular membranes; coatomer protein; coated vesicle; acyl coenzyme a; golgi apparatus; biological transport, active; active transport; adp-ribosylation factors; priority journal; article; cell compartmentation; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; hamsters |
| Journal Title: | Cell |
| Volume: | 75 |
| Issue: | 5 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1993-12-03 |
| Start Page: | 1015 |
| End Page: | 1025 |
| Language: | English |
| DOI: | 10.1016/0092-8674(93)90545-2 |
| PUBMED: | 8252615 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
