A Structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes Journal Article
| Authors: | Yu, X.; Breitman, M.; Goldberg, J. |
| Article Title: | A Structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes |
| Abstract: | Budding of COPI-coated vesicles from Golgi membranes requires an Arf family G protein and the coatomer complex recruited from cytosol. Arf is also required with coatomer-related clathrin adaptor complexes to bud vesicles from the trans-Golgi network and endosomal compartments. To understand the structural basis for Arf-dependent recruitment of a vesicular coat to the membrane, we determined the structure of Arf1 bound to the γζ-COP subcomplex of coatomer. Structure-guided biochemical analysis reveals that a second Arf1-GTP molecule binds to βδ-COP at a site common to the γ- and β-COP subunits. The Arf1-binding sites on coatomer are spatially related to PtdIns4,5P 2-binding sites on the endocytic AP2 complex, providing evidence that the orientation of membrane binding is general for this class of vesicular coat proteins. A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules. © 2012 Elsevier Inc. |
| Keywords: | chemical analysis; protein interaction; binding site; protein structure; guanosine triphosphate; membrane binding; endosome; clathrin; coatomer protein; adenosine diphosphate ribosylation factor 1; coat protein; trans golgi network |
| Journal Title: | Cell |
| Volume: | 148 |
| Issue: | 3 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2012-02-03 |
| Start Page: | 530 |
| End Page: | 542 |
| Language: | English |
| DOI: | 10.1016/j.cell.2012.01.015 |
| PROVIDER: | scopus |
| PMCID: | PMC3285272 |
| PUBMED: | 22304919 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2012" - "CODEN: CELLB" - "Source: Scopus" |
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