Binding of coatomer to Golgi membranes requires ADP-ribosylation factor Journal Article
| Authors: | Palmer, D. J.; Helms, J. B.; Beckers, C. J. M.; Orci, L.; Rothman, J. E. |
| Article Title: | Binding of coatomer to Golgi membranes requires ADP-ribosylation factor |
| Abstract: | Coatomer, a complex of seven proteins, appears to be the precursor of the coat structure of non-clathrin-coated Golgi-derived vesicles. Another component of this vesicle coat is the cytosolic protein ADP-ribosylation factor (ARF). Like coatomer, ARF appears to reversibly associate with Golgi membranes. We now report that ARF is required for coatomer binding to Golgi membranes and that myristoylated, but not non-myristoylated, ARF is the required species. We utilize an antibody directed against the β-subunit of coatomer (β-COP) to follow coatomer binding. ARF and β-COP bind stoichiometrically to Golgi membranes. ARF-dependent β-COP binding requires a membrane-associated protein, is saturable, and is enhanced in the presence of stable GTP analogues like guanosine 5′-O-(3-thiotriphosphate) (GTPγS). ARF and β-COP bind sequentially to Golgi membranes, since β-COP can be bound to reisolated membranes that had been previously incubated with ARF and GTPγS. We conclude that membrane-bound ARF confers to Golgi membranes all of the requirements for specific β-COP binding. |
| Keywords: | nonhuman; animal; animal tissue; protein binding; gtp-binding proteins; membrane proteins; brain; recombinant proteins; protein transport; immunoblotting; cattle; molecular weight; cytosol; electrophoresis, polyacrylamide gel; golgi complex; adenosine diphosphate ribosylation factor; adenosine diphosphate ribosylation; microtubule-associated proteins; intracellular membranes; guanosine 5'-o-(3-thiotriphosphate); coatomer protein; myristic acid; beta chain; golgi apparatus; binding kinetics; myristic acids; adp-ribosylation factors; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; macromolecular systems |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 268 |
| Issue: | 16 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1993-06-05 |
| Start Page: | 12083 |
| End Page: | 12089 |
| Language: | English |
| PUBMED: | 8505331 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2019 -- Source: Scopus |
