ADP-ribosylation factor and coatomer couple fusion to vesicle budding Journal Article
| Authors: | Elazar, Z.; Orci, L.; Ostermann, J.; Amherdt, M.; Tanigawa, G.; Rothman, J. E. |
| Article Title: | ADP-ribosylation factor and coatomer couple fusion to vesicle budding |
| Abstract: | The coat proteins required for budding COP-coated vesicles from Golgi membranes, coatomer and ADP-ribosylation factor (ARF) protein, are shown to be required to reconstitute the orderly process of transport between Golgi cisternae in which fusion of transport vesicles begins only after budding ends. When either coat protein is omitted, fusion is uncoupled from budding- donor and acceptor compartments pair directly without an intervening vesicle. Coupling may therefore result from the sequestration of fusogenic membrane proteins into assembling coated vesicles that are only exposed when the coat is removed after budding is complete. This mechanism of coupling explains the phenomenon of 'retrograde transport' triggered by uncouplers such as the drug brefeldin A. |
| Keywords: | membrane protein; cell free system; mitochondrial membrane; cytosol; golgi complex; adenosine diphosphate ribosylation factor; membrane vesicle; cell vacuole; electron transport; coat protein; priority journal; article |
| Journal Title: | Journal of Cell Biology |
| Volume: | 124 |
| Issue: | 4 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1994-02-15 |
| Start Page: | 415 |
| End Page: | 424 |
| Language: | English |
| DOI: | 10.1083/jcb.124.4.415 |
| PROVIDER: | scopus |
| PMCID: | PMC2119908 |
| PUBMED: | 8106543 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
