An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding Journal Article

   


Authors: Stamnes, M. A.; Craighead, M. W.; Hoe, M. H.; Lampen, N.; Geromanos, S.; Tempst, P.; Rothman, J. E.
Article Title: An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding
Abstract: We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.
Keywords: nonhuman; comparative study; polymerase chain reaction; animal cell; animal; microscopy, electron; gtp-binding proteins; membrane proteins; animalia; amino acid sequence; molecular sequence data; sequence homology, amino acid; brain; saccharomyces cerevisiae; dogs; signal peptide; carrier proteins; membrane protein; protein transport; base sequence; cattle; dna primers; cytosol; cell fusion; organelles; golgi complex; cho cell; cho cells; membrane vesicle; cell vacuole; arabidopsis; coatomer protein; coated vesicle; membrane fusion; membrane receptor; golgi apparatus; coat protein; adp-ribosylation factors; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; hamsters
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 92
Issue: 17
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1995-08-15
Start Page: 8011
End Page: 8015
Language: English
DOI: 10.1073/pnas.92.17.8011
PUBMED: 7644530
PROVIDER: scopus
PMCID: PMC41276
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029147574&doi=10.1073%2fpnas.92.17.8011&partnerID=40&md5=d309f3a3b6a036e1d1744a933c6107bd
Notes: Article -- Export Date: 28 August 2018 -- Source: Scopus
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MSK Authors
  1. James E Rothman
    120 Rothman
  2. Paul J Tempst
    324 Tempst
  3. Nina M Lampen
    11 Lampen
  4. Scott Geromanos
    16 Geromanos
  5. Mee H Hoe
    2 Hoe
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