Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors Journal Article

  

Authors:	Bremser, M.; Nickel, W.; Schweikert, M.; Ravazzola, M.; Amherdt, M.; Hughes, C. A.; Söllner, T. H.; Rothman, J. E.; Wieland, F. T.
Article Title:	Coupling of coat assembly and vesicle budding to packaging of putative cargo receptors
Abstract:	COPI-coated vesicle budding from lipid bilayers whose composition resembles mammalian Golgi membranes requires coatomer, ARF, GTP, and cytoplasmic tails of putative cargo receptors (p24 family proteins) or membrane cargo proteins (containing the KKXX retrieval signal) emanating from the bilayer surface. Liposome-derived COPI-coated vesicles are similar to their; native counterparts with respect to diameter, buoyant density, morphology, and the requirement for an elevated temperature for budding. These results suggest that a bivalent interaction of coatomer with membrane- bound ARF[GTP] and with the cytoplasmic tails of cargo or putative cargo receptors is the molecular basis of COPI coat assembly and provide a simple mechanism to couple uptake of cargo to transport vesicle formation.
Keywords:	carrier protein; nonhuman; intracellular transport; protein transport; guanosine triphosphate; lipid bilayer; golgi complex; transport kinetics; membrane vesicle; cell ultrastructure; temperature dependence; human; priority journal; article; antigen p24
Journal Title:	Cell
Volume:	96
Issue:	4
ISSN:	0092-8674
Publisher:	Cell Press
Date Published:	1999-02-19
Start Page:	495
End Page:	506
Language:	English
PUBMED:	10052452
PROVIDER:	scopus
DOI:	10.1016/S0092-8674(00)80654-6
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033582648&partnerID=40&md5=26b3a18ed9af7ad7e46512394eea571f
Notes:	Article -- Export Date: 16 August 2016 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/96069