Bidirectional transport by distinct populations of COPI-coated vesicles Journal Article
| Authors: | Orci, L.; Stamnes, M.; Ravazzola, M.; Amherdt, M.; Perrelet, A.; Söllner, T. H.; Rothman, J. E. |
| Article Title: | Bidirectional transport by distinct populations of COPI-coated vesicles |
| Abstract: | Electron microscope immunocytochemistry reveals that both anterograde- directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde- directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein. |
| Keywords: | nonhuman; pancreas; animal cell; animals; cells, cultured; cell protein; membrane proteins; intracellular transport; animalia; rat; newborn; protein transport; rats; receptor; animals, newborn; guanine nucleotide binding protein; biological transport; golgi complex; membrane vesicle; immunoelectron microscopy; coatomer protein; coated vesicles; cell-free system; golgi apparatus; receptors, peptide; microscopy, immunoelectron; proinsulin; priority journal; article |
| Journal Title: | Cell |
| Volume: | 90 |
| Issue: | 2 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1997-07-25 |
| Start Page: | 335 |
| End Page: | 349 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)80341-4 |
| PUBMED: | 9244307 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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