Sonophore-enhanced nanoemulsions for optoacoustic imaging of cancer Journal Article


Authors: Roberts, S.; Andreou, C.; Choi, C.; Donabedian, P.; Jayaraman, M.; Pratt, E. C.; Tang, J.; Pérez-Medina, C.; de la Cruz, M. J.; Mulder, W. J. M.; Grimm, J.; Kircher, M.; Reiner, T.
Article Title: Sonophore-enhanced nanoemulsions for optoacoustic imaging of cancer
Abstract: Optoacoustic imaging offers the promise of high spatial resolution and, at the same time, penetration depths well beyond the conventional optical imaging technologies, advantages that would be favorable for a variety of clinical applications. However, similar to optical fluorescence imaging, exogenous contrast agents, known as sonophores, need to be developed for molecularly targeted optoacoustic imaging. Despite numerous optoacoustic contrast agents that have been reported, there is a need for more rational design of sonophores. Here, using a library screening approach, we systematically identified and evaluated twelve commercially available near-infrared (690-900 nm) and highly absorbing dyes for multi-spectral optoacoustic tomography (MSOT). In order to achieve more accurate spectral deconvolution and precise data quantification, we sought five practical mathematical methods, namely direct classical least squares based on UV-Vis (UV/Vis-DCLS) or optoacoustic (OA-DCLS) spectra, non-negative LS (NN-LS), independent component analysis (ICA) and principal component analysis (PCA). We found that OA-DCLS is the most suitable method, allowing easy implementation and sufficient accuracy for routine analysis. Here, we demonstrate for the first time that our biocompatible nanoemulsions (NEs), in combination with near-infrared and highly absorbing dyes, enable non-invasive in vivo MSOT detection of tumors. Specifically, we found that NE-IRDye QC1 offers excellent optoacoustic performance and detection compared to related near-infrared NEs. We demonstrate that when loaded with low fluorescent or dark quencher dyes, NEs represent a flexible and new class of exogenous sonophores suitable for non-invasive pre-clinical optoacoustic imaging. © 2018 The Royal Society of Chemistry.
Keywords: diagnosis; biocompatibility; fluorescence quenching; fluorescence imaging; clinical application; infrared devices; principal component analysis; independent component analysis; high spatial resolution; least squares approximations; photoacoustic effect; optoacoustic tomography; classical least squares; independent component analyses (ica); optical fluorescence; optical imaging technology; spectral deconvolution
Journal Title: Chemical Science
Volume: 9
Issue: 25
ISSN: 2041-6520
Publisher: Royal Society of Chemistry  
Date Published: 2018-07-07
Start Page: 5646
End Page: 5657
Language: English
DOI: 10.1039/c8sc01706a
PROVIDER: scopus
PMCID: PMC6049522
PUBMED: 30061998
DOI/URL:
Notes: Article -- Export Date: 1 August 2018 -- Source: Scopus
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MSK Authors
  1. Moritz Florian Kircher
    42 Kircher
  2. Jan Grimm
    55 Grimm
  3. Thomas Reiner
    71 Reiner
  4. Chrysafis   Andreou
    10 Andreou
  5. Edwin C Pratt
    2 Pratt
  6. Crystal Choi
    1 Choi