Virtual biopsy of the joint tissues using near-infrared, reflectance confocal microscopy - A pilot study Journal Article
| Authors: | Campo-Ruiz, V.; Patel, D.; Anderson, R. R.; Delgado-Baeza, E.; Gonzalez, S. |
| Article Title: | Virtual biopsy of the joint tissues using near-infrared, reflectance confocal microscopy - A pilot study |
| Abstract: | Standard noninvasive imaging techniques applied to joints provide gross morphological features, insufficient for assessing histological detail. On the other hand, biopsying is invasive, time consuming, and may involve unwanted processing artifacts. Near-infrared reflectance confocal microscopy is a technique that allows serial, high-resolution optical sectioning through intact tissues without employing exogenous fluorescent stains. The aim of this work was to evaluate the potential utility of near-infrared reflectance confocal microscopy for providing immediate histological information on meniscus, articular cartilage, epiphyseal plate, bone, muscle, and tendon. Images from near-infrared reflectance confocal microscopy were compared with mirror routine histology sections. Characteristic architectural features were readily visualized in the three dimensions of space. Additionally, the use of experimental contrast agents highlighted the localization of nuclei. Limitations include penetration depth and minor optical artifacts. In conclusion, near-infrared reflectance confocal microscopy is a useful technique for immediate, nondestructive, serial "virtual" sectioning through intact tissues, being thus a potential adjunct to current imaging techniques in orthopedics. © 2006 Wiley-Liss, Inc. |
| Keywords: | nonhuman; mouse; animals; mice; animal tissue; confocal microscopy; microscopy, confocal; fluorescent dye; diagnostic imaging; biopsy; histology; morphology; tissue section; medical imaging; pilot study; pilot projects; three dimensional imaging; rat; bone; knee; contrast medium; artifact; cell nucleus; non invasive procedure; orthopedics; optical resolution; cytodiagnosis; muscle; joints; mice, hairless; epiphysis plate; cartilage; virtual reality; microscope; virtual biopsy; knee meniscus; ligament; mice, inbred hrs; meniscus; articular cartilage; joint biopsy; near infrared reflectance confocal microscopy; tendon; spectroscopy, near-infrared |
| Journal Title: | Microscopy Research and Technique |
| Volume: | 69 |
| Issue: | 10 |
| ISSN: | 1059-910X |
| Publisher: | Wiley Liss |
| Date Published: | 2006-10-01 |
| Start Page: | 794 |
| End Page: | 798 |
| Language: | English |
| DOI: | 10.1002/jemt.20348 |
| PUBMED: | 16758475 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 4 June 2012" - "CODEN: MRTEE" - "Source: Scopus" |
