Real-time reflectance confocal microscopy, a noninvasive tool for in vivo quantitative evaluation of comedolysis in the rhino mouse model Journal Article
| Authors: | Nakano, K.; Kiyokane, K.; Benvenuto-Andrade, C.; Gonzalez, S. |
| Article Title: | Real-time reflectance confocal microscopy, a noninvasive tool for in vivo quantitative evaluation of comedolysis in the rhino mouse model |
| Abstract: | Background: Near-infrared reflectance confocal microscopy (RCM) is a noninvasive tool that provides real-time images of thin virtual horizontal tissue sections. Aims/Methods: We have used a rhino mouse model in combination with topical application of all-trans-retinoic acid and all-trans-retinol to investigate the usefulness of RCM as a noninvasive imaging tool to evaluate comedolysis in vivo and over time. Optical images were correlated with routine histology. Results: Our results demonstrate that RCM in vivo can visualize the process of transformation of utriculi (pseudocomedones) towards a normal-appearing follicular structure during retinoid treatment. The retinoic acid intervention group showed a dose-related response, while the vehicle-treated group did not show utricular changes. Conclusions: RCM represents a useful tool for in vivo morphological and quantitative evaluation of skin utriculi over time and could be used as an adjunct tool to histopathological techniques for comedolysis studies. Copyright © 2007 S. Karger AG. |
| Keywords: | controlled study; dose response; drug efficacy; nonhuman; mouse; animal; animals; mice; animal tissue; reflectance confocal microscopy; confocal microscopy; microscopy, confocal; animal experiment; animal model; in vivo study; pathology; disease model; quantitative analysis; disease models, animal; retinol; retinoic acid; vitamin a; comedo; tretinoin; all-trans-retinoic acid; all-trans-retinol; acne vulgaris; rhino mouse, comedones |
| Journal Title: | Skin Pharmacology and Physiology |
| Volume: | 20 |
| Issue: | 1 |
| ISSN: | 1660-5527 |
| Publisher: | S. Karger AG |
| Date Published: | 2007-01-01 |
| Start Page: | 29 |
| End Page: | 36 |
| Language: | English |
| DOI: | 10.1159/000096169 |
| PUBMED: | 17035719 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 7" - "Export Date: 4 June 2012" - "CODEN: SPPKC" - "Source: Scopus" |
