Real-time reflectance confocal microscopy, a noninvasive tool for in vivo quantitative evaluation of comedolysis in the rhino mouse model Journal Article


Authors: Nakano, K.; Kiyokane, K.; Benvenuto-Andrade, C.; Gonzalez, S.
Article Title: Real-time reflectance confocal microscopy, a noninvasive tool for in vivo quantitative evaluation of comedolysis in the rhino mouse model
Abstract: Background: Near-infrared reflectance confocal microscopy (RCM) is a noninvasive tool that provides real-time images of thin virtual horizontal tissue sections. Aims/Methods: We have used a rhino mouse model in combination with topical application of all-trans-retinoic acid and all-trans-retinol to investigate the usefulness of RCM as a noninvasive imaging tool to evaluate comedolysis in vivo and over time. Optical images were correlated with routine histology. Results: Our results demonstrate that RCM in vivo can visualize the process of transformation of utriculi (pseudocomedones) towards a normal-appearing follicular structure during retinoid treatment. The retinoic acid intervention group showed a dose-related response, while the vehicle-treated group did not show utricular changes. Conclusions: RCM represents a useful tool for in vivo morphological and quantitative evaluation of skin utriculi over time and could be used as an adjunct tool to histopathological techniques for comedolysis studies. Copyright © 2007 S. Karger AG.
Keywords: controlled study; dose response; drug efficacy; nonhuman; mouse; animal; animals; mice; animal tissue; reflectance confocal microscopy; confocal microscopy; microscopy, confocal; animal experiment; animal model; in vivo study; pathology; disease model; quantitative analysis; disease models, animal; retinol; retinoic acid; vitamin a; comedo; tretinoin; all-trans-retinoic acid; all-trans-retinol; acne vulgaris; rhino mouse, comedones
Journal Title: Skin Pharmacology and Physiology
Volume: 20
Issue: 1
ISSN: 1660-5527
Publisher: S. Karger AG  
Date Published: 2007-01-01
Start Page: 29
End Page: 36
Language: English
DOI: 10.1159/000096169
PUBMED: 17035719
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 7" - "Export Date: 4 June 2012" - "CODEN: SPPKC" - "Source: Scopus"
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  1. Salvador Gonzalez Rodriguez
    203 Rodriguez